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  • 1
    Electronic Resource
    Electronic Resource
    The capsule and slime polysaccharides of the wild type and a phage resistant mutant ofRhodopseudomonas capsulata St. Louis (1984)
    Springer
    Archives of microbiology 139 (1984), S. 38-43 
    Details
    ISSN: 1432-072X
    Keywords: Rhodopseudomonas capsulata ; Phage resistant mutant ; Capsule ; Slime
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rhamnose, galactose and pyruvic acid containing polysaccharide (capsule) together with the peptidoglycan was isolated fromRhodopseudomonas capsulata St. Louis as the insoluble sediment after sodium dodecyl sulfate extraction of cell envelope fractions. Treatment with pronase E separated the soluble polysaccharide from the insoluble peptidoglycan. After lysozyme-digestion, both the capsule polysaccharide and peptidoglycan were soluble. The capsule was also accumulated in the combined interphase/phenol-phase of hot phenol-water extracts of whole cells. Again, the capsule and peptidoglycan were sedimented together as long as no pronase E-treatment was performed. With the phage-resistant mutant (R. capsulata St. Louis RC1-), no capsule polysaccharide was obtained in the combined interphase/phenol phase. An acidic polysaccharide (slime) different from the capsule in composition and serology was obtained by Cetavlon fractionation of hot phenol/water extracts of cells of both the wild-type and the mutant cells. It was shown to consist mainly of rhamnose, glucosamine and galacturonic acid. The use of O/K-antisera and of capsule polysaccharideantisera allowed a separate visualization of the capsule and slime layers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692709
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  • 2
    Electronic Resource
    Electronic Resource
    Different polysaccharides in the external layers (capsule and slime) of the cell envelope of Rhodopseudomonas capsulata Sp11 (1983)
    Springer
    Archives of microbiology 136 (1983), S. 291-296 
    Details
    ISSN: 1432-072X
    Keywords: Rhodopseudomonas capsulata ; Capsule ; Slime ; Polysaccharides ; External layers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rhamnose, galactose, 3-amino-3,6-dideoxygalactose, an unknown amino sugar and galacturonic acid, it represents very likely the capsule of R. capsulata Sp11. Polysaccharide I has a serological specificity different from that of polysaccharide II as shown by immunoprecipitation using antisera against living cells. Polysaccharide II, but not polysaccharide I, reacts in antiserum against heattreated cells (100°C, 2.5 h). Whole cells are agglutinated in the antisera against living but not in those against heattreated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425219
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  • 3
    Electronic Resource
    Electronic Resource
    Lipopolysaccharides of two strains of the phototrophic bacterium Rhodopseudomonas capsulata (1983)
    Springer
    Archives of microbiology 134 (1983), S. 212-216 
    Details
    ISSN: 1432-072X
    Keywords: Rhodopseudomonas capsulata ; Lipopolysaccharide ; Lipid A ; l-Acofriose ; 3-Oxo-myristic acid ; Serological activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipopolysaccharides of Rhodopseudomonas capsulata strains St. Louis (ATCC 23782) and Sp 11 both contain l-acofriose, rhamnose, glucose and glucosamine as the main sugar constituents. 2-Keto-3-deoxyoctonate and neuraminic acid were tentatively identified. The fatty acid spectrum found with both strains comprises 3-OH−C10 and C12:1 (ester-linked) and 3-oxo-C14 (amide-linked). Isolated lipid A from strain Sp 11 contains glucosamine, glucosamine-phosphate and the total of the fatty acids of the lipopolysaccharide. Methylation analysis of the degraded polysaccharide of this lipopolysaccharide shows l-acofriose in both terminal and 1→2 chain-linked positions in a 1:4 molar ratio. Rhamnose is exclusively chain-linked (1→2), glucose is both terminally and chain-linked (1→6) in a 1:1 molar ratio. The serological activity of the lipopolysaccharide of both the R. capsulata strains is low in antisera against living or heat-killed cells when tested by passive hemagglutination, Ouchterlony immunoprecipitation or gel-immunoelectrophoresis. No crossreaction was observed among the lipopolysaccharides of R. capsulata strains St. Louis, Sp 11 and 37b4 in immunoprecipitation. Lipopolysaccharide of strain Sp 11 was found to lack lethal toxicity in galactosamine-sensitized mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407760
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