ISSN:
1617-4623
Keywords:
Retrotransposons
;
Reverse transcription
;
Ty elements
;
Yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Transposition of the yeast transposable element, Ty, has been shown to require a reverse transcription process. By analysing the extrachromosomal Tyspecific nucleic acid molecules associated with overproduced Ty virus-like particles (Ty-VLPs), we identified several reverse transcribed cDNA strands. Most of them resemble the characteristic intermediates of the reverse transcription process described for authentic retroviruses: a (−) strong-stop DNA strand covalently bound to an RNA primer, two elongated (−) strands with one or two long terminal repeat (LTR) sequences and a (+) strong-stop DNA. Surprisingly, complete (+) strands and full-length linear duplex Ty DNA could not be detected. The structural features of two additional (÷) strands may indicate some differences between the mechanisms of (+) strand synthesis in Ty and other retrotransposons or retroviruses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273598
Permalink
