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  • 1
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 195-199 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Linkage map ; Helianthus annuus ; Sunflower cDNAs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed linkage map of Helianthus annuus was constructed based on segregation at 234 RFLP loci, detected by 213 probes, in an F2 population of 289 individuals (derived from a cross between the inbred lines HA89 and ZENB8). The genetic markers covered 1380 centiMorgans (cM) of the sunflower genome and were aranged in 17 linkage groups, corresponding to the haploid number of chromosomes in this species. One locus was found to be unlinked. Although the average interval size was 5.9 cM, there were a number of regions larger than 20 cM that were devoid of markers. Genotypic classes at 23 loci deviated significantly from the expected ratios (1∶2∶1 or 3∶1), all showing a reduction in the ZENB8 homozygous class. The majority of these loci were found to map to four regions on linkage groups G, L and P.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220877
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of Helianthus annuus L. 1. Restriction fragment length polymorphism between inbred lines of cultivated sunflower (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 435-441 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Helianthus annuus ; Cytoplasmic male sterility (CMS) ; Restorer ; Maintainer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA and PstI genomic clones have been used to assess levels of restriction fragment length polymorphism (RFLP) in Helianthus annuus and to determine the inter-relationships between a diverse set of 24 inbred lines. Of the cDNA clones screened 45% were useful as RFLP probes, compared to less than 20% from the PstI library, which showed high levels of redundancy for high copy sequences. Fifty-seven low-copy DNA probes (23 PstI and 34 cDNA clones) were used to fingerprint 12 maintainer (B) lines and 12 restorer (R) lines. The average number of RFLP variants per probe was found to be 3.2, with a mean polymorphic index of 0.49, indicating that high levels of nuclear DNA polymorphism are to be found in cultivated sunflower. Cluster and principal coordinate analysis of the fingerprinting data clearly separated the maintainer and restorer lines, but there was a degree of association between 2 unbranched R-lines and the B-line germ plasm pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225378
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    Paper (German National Licenses)
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