ISSN:
1432-0878
Keywords:
Reproductive system, male
;
Ductuli efferentes
;
Epithelial cells
;
Cell culture
;
Monolayer cultures
;
Man
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The culture of epithelial cells lining human efferent ducts, obtained from prostatic carcinoma patients, is described. Ciliated cells were observed to beat for at least one month on plastic. On pervious filters low cuboidal cells characterized the monolayers. Cells comprising monolayers over the filter were 5 to 9 μm in height whereas taller cells were found over the original fragments (14 μm). Some non-ciliated cells contained dark and light vacuoles, others were found to lack them. Both non-ciliated and ciliated cells maintained tight junctional complexes restricting the paracellular movement of horseradish peroxidase. Both types of cultured cells exhibited fluid-phase and adsorptive endocytosis from both apical and basal surfaces. It is reported for the first time that the monolayers form high resistance barriers (150 Ω cm2) that prevent the apical medium from draining to the basal compartment over 24 h.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00645053
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