ISSN:
1432-072X
Keywords:
Streptomyces
;
Regulatory protein
;
Factor C
;
Monoclonal antibody
;
ELISA
;
Immunoblotting
;
Factor C-like antigen
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen in bacterial specimens were developed. Factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34 000 or 70 000 in mycelial homogenates. Most of factor C was found excreted into the cultivation medium. The quantity of factor C antigen in different Streptomyces strains showed great variation. Amy + strains were usually good producers of factor C while Amy − were not. This was consistent with our assumption that factor C was an inducer of reproductive phase in Streptomyces.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249182
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