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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical differences between carrot inbreds differing in plant regeneration potential (1991)
    Springer
    Plant cell reports 10 (1991), S. 152-155 
    Details
    ISSN: 1432-203X
    Keywords: Daucus ; Regeneration ; Ethylene ; Protein ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultures derived from domestic carrot (Daucus carota L.) inbreds were found to vary with respect to regeneration potential as measured by the production of somatic embryos in suspension cultures. A number of biochemical parameters previously reported to distinguish embryogenic from non-embryogenic cultures of other species were measured in these carrot cell lines. Ethylene production was found to be inversely related to regeneration potential. The cell line producing the greatest number of somatic embryos exhibited the lowest rate of ethylene biosynthesis, even when grown on 2, 4-D-containing maintenance medium. A specific isozyme of acid phosphatase was associated with embryogenic calli. Proteins visualized by SDS-PAGE did not discriminate between embryo-forming and proliferating calli in all inbreds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232048
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets (1998)
    Springer
    Plant cell reports 17 (1998), S. 726-730 
    Details
    ISSN: 1432-203X
    Keywords: Key words Garlic ; Allium sativum L. ; Root segments ; Regeneration ; Picloram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 4,5 μm 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μm 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μm 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050473
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    Paper (German National Licenses)
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