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  • Aquaculture  (49)
  • Fisheries  (49)
  • Rainbow trout  (24)
  • 1
    Publikationsdatum: 2021-05-19
    Beschreibung: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Sperm Cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Oncorhynchus mykiss ; Breeding ; Genetic
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 54pp.
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2021-05-19
    Beschreibung: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Population ; Survey ; Coldwater ; Egg ; Temperature ; Sperm ; Motility ; Fertilization
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 51pp.
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/21909 | 18721 | 2018-01-16 10:47:13 | 21909 | Iranian Fisheries Science Research Institute
    Publikationsdatum: 2021-07-04
    Beschreibung: In the present study, hydrolysed protein of viscera and head of Silver carp (Hypophthalmichthys molitrix) was compared using Alcalase enzyme and internal tissue enzymes at 2 and 4 hours. The result indicated that product by Alcalase (Treatment 1) had significantly higher protein and rate of hydrolysates than that produced by internal tissue enzymes (Treatment 2). So, the highest mean (±SD) protein (68.10±1.33) was related to treatment 1-head (with Alcalase enzyme) after 4 hours and the highest rate of hydrolysates (29.36±1.35) was related to treatment 1-head (with Alcalase enzyme) after 4 hours. The result indicated that rate of hydrolysates raised as time of hydrolysates increased. However the intensity and rate of hydrolysates is reduced. The highest rate of hydrolysates occurred at 120 minutes in the first. This mode was similar for two treatments. The result can be considered as the Alcalase was preferred to internal enzyme.
    Schlagwort(e): Chemistry ; Fisheries ; Biotechnology ; Fish processing ; Fish wast ; Hypophthalmichthys molitrix ; protein ; Silver carp ; tissue ; enzymes ; Iran
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 55-62
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
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    In:  http://aquaticcommons.org/id/eprint/21938 | 18721 | 2018-01-17 12:40:29 | 21938 | Iranian Fisheries Science Research Institute
    Publikationsdatum: 2021-07-05
    Beschreibung: The changes in chemical, microbial and quality sensory of fillets for silver carp fish treated with black cumin extract during refrigerated storage were investigated. The fish were divided into two groups. First group was dipped in black cumin extract with concentration of 1% and received the highest score in sensory evaluation. Then the fishes were wrapped in polyethylene package. The second group, as the control samples, were wrapped in polyethylene package after dipping in distilled water. All treatments were stored at refrigerator temperature (1±4°C). The microbial tests including total viable count, psychrotrophic count and chemical tests including peroxide index, thiobarbituric acid, free fatty acid value and total volatile basic nitrogen, with sensory evaluation, were done at 4°C over a period of 15 days. The results showed that the black cumin extract delayed lipid oxidation and protein analyses significantly in treated fishes. Psycrotrophic bacteria and total viable count of samples being treated with black cumin extract were maintained lower than the proposed acceptable limit (7 log cfu/g). In comparison to the control samples, microbial spoilage significantly decreased in treated samples. Furthermore, according to sensory analysis, the treatment with black cumin extract led to high quality during storage.The findings indicated that black cumin exerts had strong antioxidant and antibacterial impacts on silver carp fish, such that the shelf life of fillets being treated with black cumin were 2.5 times more than that of control samples during storage in refrigerator.
    Schlagwort(e): Biology ; Fisheries ; Silver carp ; black cumin extract ; shelf life ; Hypophthalmichthys molitrix ; fish ; Chemical ; microbial ; sensory ; Nigella sativa L. ; Black cumin ; refrigerator ; fillet ; Iran
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 71-84
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25446 | 18721 | 2018-09-25 18:05:26 | 25446 | Iranian Fisheries Science Research Institute
    Publikationsdatum: 2021-07-16
    Beschreibung: In this project, the nutritional effects of Sargassum illicifolium Chabahar bay-Oman Sea, on growth and survival rates of whiteleg shrimp (Litopenaeus vannamei) were studied. The seaweed collected from 6 coastal area, rinsed, dried, powdered and measured the nutritional values in laboratory for surveying statistically. According to the high nutritional value of Tis coastal seaweed, this variate seaweed powder, replaced with protein resources (fish meal and Soy and Wheat) of whiteleg shrimp feed which was formulated by Havorash feed factory of Boshehr in four treatments (A: as control without any replacement) B: with 5%, C: 10 % and D: 15% seaweed replacement, each with three replicates in order to obtain isonitrogenus 33% CP., and Isocaloric (13% fat and 15% carbohydrate) feed. The weighed milled ingredients were carefully mixed using a laboratory food mixer. The mixtures were primed with 30% hot water to yield a suitable pulp. Wet diets were made into 2 mm pellet size and dried at 40 °C in a drying cabinet and maintained in standard condition which was used according to daily need shrimp, calculated by each 10 days biometry. Water stability and absorbtion capacity of the pellets in sea water were measured and compared statistically. Juvenile shrimps (Initial body weight =3 g) brought from Jask hatchery, acclimazed for one week in Chabahar hatchery condition and feeding daily 3-5% body weight. Abiotical parameters and weight and length biometrics were measured two days and 10 days, respectively. After 45 days and final biometry, FCR, CF, SGR, caracas analysis, muscle colourimetery with HPLC were done, tasted with pp Plot for determining the parametric data and statistically differences using one – way ANOVA, Duncan test of SPSS software. The Tis coastal seaweed with 9.8% CP, 2% lipid and 23% carbohydrate had higher nutritional value compared to the other gathered seaweed. Also amino acid and fatty acid profiles, vitamins and minerals were measured in all seaweed samples each, with three replications. As result, the water stability of D feed treatment in seawater (98%) and C (97%) had statistical differences with A and B (95% stability) (P〈0.05). Water absorption capacity of feeds after one hour immersion in seawater showed significance difference between D (110%) and three others, C(100%), B(85% and control(80%) (P〈0.05). Shrimp growth data, after the end of experiment revealed that seaweed feed treatments had no any differences with control group significantly. However, the absolute growth rate datas of D treatment were higher than others and the lower weight and lenght were measured in control group shrimp. FCR had difference between seaweed treatments and control statistically. There are no any differences between caracas lipid treatments (P〉0.05) but Cholestrol content of, showed differences between all, significantly (P〈0.05) which was the highest (121.68±12.12) in D and the lowest in A (147.92±11.02). Feed treatment D and C performed colour changes pink partial orang and pink in shrimp muscle with no any difference compared to white and none colour in shrimp were fed B and A feed treatments . It seems this colour changing can be playing a major role in market acceptability.
    Schlagwort(e): Aquaculture ; Iran ; Oman Sea ; Sargassum illicifolium ; Biochemical composition ; Replacement ; Litopenaeus vannamei ; Growth performance
    Repository-Name: AquaDocs
    Materialart: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 87
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25512 | 18721 | 2018-10-05 15:26:26 | 25512 | Iranian Fisheries Science Research Institute
    Publikationsdatum: 2021-07-16
    Beschreibung: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Schlagwort(e): Aquaculture ; Iran ; Yasouj ; Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Coldwater ; Temperature ; Fertilization
    Repository-Name: AquaDocs
    Materialart: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 51
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2021-05-19
    Beschreibung: A sharp decline in natural population of Caspian brown trout lead to implementing of restocking program in Iran. Along the stock recovery program, the artificial production of this species has been attracted too much attention in recent years. The objective of the study is to assess the effects of protein and energy levels of diet on fish performance, growth efficiency of Caspian brown trout in order to develop a proper diet for production of this species. Six experimental diets were formulated by replacing casein as a pure protein source with fat as an energy source in the diets according to a 3×2 factorial design. The diet was assigned to 18 tanks with 50 fish each, with three replicated for each diet. The experiment was conducted for 8-weeks with Caspian brown trout with an initial body weight of around 7 g. Caspian brown trout demonstrated a better FCR and a larger SGR at lower protein levels (45 and 50%) in comparison to high protein level (55%; P〈0.05). Protein efficiency ratio improved by a decrease in protein content of the diet (P〈0.05), but energy content of diets does not affect any growth related parameter such as FCR, SGR and PER. There was also no interaction between protein and energy levels in the growth related parameters, suggesting the effect of protein on the growth parameters in Caspian brown trout did not depend on energy levels of diet. In conclusion diet containing 50% protein can support the maximum growth. High dietary energy level of the diet dose in cooperate in protein sparing effect.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Caspian brown trout ; Artificial production ; Growth ; Diet ; Protein ; Energy ; Weight ; FCR ; SGR ; Salmo trutta caspius ; Aquaculture
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 40pp.
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Publikationsdatum: 2021-05-19
    Beschreibung: In order to perform the project, 446 samples of rainbow trout (Onchorhynchus mykiss) from 24 different regions in Iran were collected. About 2-3 g of caudal fin samples was collected from each specimen and preserved in absolute ethyl alcohol and then transferred to the genetic laboratory. Genomic DNA was extracted using the phenol-chloroform method and then DNA content and quality was determined using spectrophotometry and agarose gel electrophoresis, respectively. Polymerase Chain Reaction (PCR) of genomic DNA fin samples was carried out using 10 pairs of microsatellite primers. All PCR products were electrophoresed on 6% polyacrylamide gel and stained with silver nitrate. Following the scoring of alleles, all parameters including allelic frequency, effective number of allele, observed and expected heterozygosity, shanon index, measurement of similarity and genetic distance and Hardy-Weinberg equilibrium, Fst , Rst and gene flow were calculated using AMOVA analysis in the GenAlex and Popgene programs. The results showed that 8 pairs of microsatellite primers were polymorphic. In total, 50 alleles were determined with the range size of 64-280 bp. The locus omyf had maximum number of allele (26) and loci OTSG 474 and Strurruta58 had minimum number of allele (5). The observed heterozygosity was between 0.86 and 0.964. Hardy-Weinberg departure was observed for all loci from farms 18, 15, 4, E20 and 21 and were disequilibrium (P〈0.05). The farms 14, 8, 7 and 6 were equilibrium at 3 loci, but showed disequilibrium in other loci. The other farms were equilibrium at 1 or 2 loci and disequilibrium at 8 or 9 loci. The FST results showed that maximum FST (0.24) were between farms 1 and 11in which had minimum of gene flow (3.7). Minimum FST (0.04) were between farms 8 and 9 in which had maximum of gene flow (346). Based on the results of AMOVA analysis, significant differences were detected between all farms (P〈0.01). Furthermore, based on Nei 's standard (1972) maximum genetic distance (0.89) were observed between farms 2 and 11 and maximum genetic similarity (0.15) were detected between farms 3 and 4. This result suggests that the unique genetic variation of rainbow trout in hatchery farms of Iran represents a highly valuable genetic resource and provide useful information for creating a based population in the future breeding programs.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Genetic ; Population ; Rainbow trout ; Onchorhynchus mykiss ; Brood stocks ; Microsatellite ; Samples ; Specimens ; DNA ; PCR ; ANOVA
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 78pp.
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2021-05-19
    Beschreibung: This survey was designed in the Helleh region, to understand effects of aquaculture industry on coastal waters of Busher, in 2000. Sediment and water samples were collected from effluent canal, influent canal and sea during the 7 month period (harvest and post harvest season). The variations of selected water quality parameters were controlled monthly. Results show that, in effluent canal, estimated parameters of water was higher than other stations every month, and tend to decreased after being discharged into the sea and got closed to normal level. Comparison of above data with permit able range of municipal and aquaculture waste and comparison of present data with data of normal condition of region, show that, aquaculture industry of Helleh region has not had any obvious negative effect on the coastal waters of Bushehr, in the culture year of 2000.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Survey ; Aquaculture ; Sediment ; Samples ; Culture ; Coastal waters ; Shrimp ; Parameters ; Water quality ; Comparison
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 65pp.
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Publikationsdatum: 2021-05-19
    Beschreibung: The effect of essential fatty acids (EFA) and vitamin C-enriched Artemia nauplii on growth, survival, and resistance to temperature (high) stress in rainbow trout larvae reared in tanks were investigated. The larvae (average weight 120.43mg±13.5) were fed 6 times daily starting at the onset of exogenous feeding for 1 week. Triplicate groups of fish were offered one of four treatments (1) commercial starter food for rainbow trout، (2) newly hatched Artemia nauplii (unenriched) ،(3) highly unsaturated fatty acid (HUFA) + vitamin C-enriched Artemia nauplii and (4) combination of 10 % HUFA+ vitamin C enriched nauplii and commercial starter food. After 1 week, all groups of fish were switched to the commercial diet for an additional period of 3 weeks. Statistical analysis of growth after the first week and at the end of the experiment, showed that growth of larvae in various treatments was significantly different (P〈0.05). After 4 weeks, the larvae in treatment 3 with the average weight of 657.50 ± 57.93 mg had the highest body weight (P〈0.05). The highest percentage of survival (96%) was observed in treatment 3 (P〈0.05). Proximate compositions of trout larvae after one week feeding with experimental diets showed that the protein in the larvae of treatments 3 and 4 was significantly different compared to other treatments (P〈0.05). The best result of resistance to temperature (up to 24ºC) was observed in larvae reared on treatment 3 with 91.34 ± 1.52 percent (P〈0.05).
    Beschreibung: Published
    Schlagwort(e): Artemia nauplii ; Rainbow trout ; Essential fatty acid ; Vitamin C ; Oncorhynchus mykiss ; Stress resistance ; Temperature ; Growth ; Survival ; Feeding ; Larvae ; Performance
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.557-569
    Standort Signatur Erwartet Verfügbarkeit
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