ISSN:
1617-4623
Keywords:
Key words Complementary RNA
;
Cosuppression
;
RNA turnover
;
Transgenic plant
;
Transient expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the neomycin phosphotransferase II (nptII) transgenes, whereas HOlo2 plants, homozygous for a single T-DNA insert, expressed the nptII genes normally. Transient expression of nptII genes newly introduced into leaves of both the HOlo2 and nptII-silenced HOlo1 plants was downregulated only in the silenced background. Different chimeric β-glucuronidase (gus) genes with parts of the nptII transgene inserted in sense or antisense orientation into the 3′-untranslated region, which encoded transcripts that had homology or complementarity to nptII transcripts, showed reduced transient expression specifically in nptII-silenced tissue. Therefore, we conclude that RNAs of both polarities are targets for PTGS-induced RNA degradation, which supports the notion that double-stranded RNA acts as an inducing signal for silencing.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008700
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