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  • 1
    Electronic Resource
    Electronic Resource
    Detection of genetic diversity and selective gene introgression in coffee using RAPD markers (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 934-940 
    Details
    ISSN: 1432-2242
    Keywords: Coffee ; Diversity ; RAPDs ; Gene introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RAPD (randomly amplified polymorphic DNA) markers generated by arbitary decamers have been successfully employed to detect genetic polymorphisms between coffee species and between Coffea arabica genotypes. The RAPD profiles were used to construct dendrograms and these were consistent with the known history and evolution of Coffea arabica. Material originating from Ethiopia and the arabica sub-groups — C. arabica var. typica and C. arabica var. bourbon — were clearly distinguished. RAPD analysis therefore reflects morphological differences between the sub-groups and the geographical origin of the coffee material. Species-specific amplification products were also identified, but, more importantly, amplification products specific to C. canephora were identified in two C. arabica genotypes, Rume Sudan and Catimor 5175. This diagnostic product is therefore indicative of interspecific gene flow in coffee and has biological implications for selective introgressive hybridisation in coffee. Our study demonstrates the power of the polymerase chain reaction technology for the generation of genetic markers for long-lived perennial tree and bush crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225787
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of the extent of genetic variation in mahoganies (Meliaceae) using RAPD markers (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 504-508 
    Details
    ISSN: 1432-2242
    Keywords: RAPDs ; Mahoganies ; Genetic variation ; Conservation ; Genetic Improvement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Despite the economic importance of mahoganies (Meliaceae) little is known of the pattern of genetic variation within this family of tropical trees. We describe the application of a polymerase chain reaction (PCR)-based polymorphic DNA assay procedure random amplified polymorphic DNAs (RAPDs) to assess the extent of genetic variation between eight mahogany species from four genera. Pronounced genetic differentiation was found between the species and genera. There was a clear separation of Cedrela odorata from the other species, with 95% of the variable amplification products differing, whereas Lovoa trichilioides, Khaya spp. and Swietenia spp. were more closely grouped. These results are consistent with the current taxonomic viewpoint. A number of markers were found to be diagnostic for particular species, which could be of value in determining the status of putative hybrids. The application of RAPDs to the study of genetic variation in mahoganies is discussed in the context of developing genetic conservation and improvement strategies for these species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225387
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers linked to genetic factors controlling the milling energy requirement of barley (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 314-320 
    Details
    ISSN: 1432-2242
    Keywords: RAPDs ; DHs ; Bulked segregant analysis ; QTLs ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Doubled haploid (DH) populations of barley have been used in combination with PCR-based polymorphic-assay procedures to identify molecular markers linked to genes controlling the milling energy requirement of the grain. Milling energy (ME) is a quantitative trait and locating individual quantitative trait loci (QTLs) involved the construction of bulks by combining DNA from DH families representing the extreme members of the distribution for ME. In addition, the individuals had alternative alleles at theRrn2 locus that has previously been shown to be linked to an ME QTL. The DNA bulks were screened with Randomly Amplified Polymorphic DNA (RAPD) markers and polymorphic amplification products tested for linkage to genes influencing the expression of ME in a DH population. Several markers were identified which are linked to a QTL controlling ME and the recombination fraction determined by maximum likelihood procedures. The results indicate that DHs in combination with RAPDs and bulked segregant analysis provide an efficient method for locating QTLs in barely. Furthermore, this approach is applicable to mapping other QTLs in a range of organisms from which DH or recombinant inbred lines can be extracted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01184917
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