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  • Fe protein  (1)
  • Pulmonate osphradium  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 118 (1971), S. 168-189 
    ISSN: 1432-0878
    Keywords: Pulmonate osphradium ; Planorbarius ; Osphradial epithelium ; Osphradial ganglion ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cellular organisation of the osphradium of Planorbarius as revealed by the previously described light microscope studies is confirmed in the present study. A third epithelial cell type, the basal cell, is described. Perception is carried out by dendritic processes from osphradial sensory neurones, forming free nerve-endings in the sensory region of the osphradial epithelium. Ciliated cells and secretory cells of the osphradial epithelium are concerned with the transport of material in the osphradial canal. Many of the ultrastructural features of molluscan central ganglia are present in the osphradial ganglion, including inter-axonal chemical synapses. Neuromuscular junctions are present in the sheath surrounding the organ and these may be involved in a behavioural response of the organ. The need for further electrophysiological studies is emphasised.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Heterocysts ; Nitrogenase ; Fe protein ; nif gene expression ; Oxygen sensitivity ; Sheath material
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vegetative cells and heterocysts of the filamentous desiccation-tolerant cyanobacteriumNostoc commune HUN retain their ultrastructural organisation and the integrity of their intra- and extracellular membranes after two years of desiccation and subsequent rehydration. Immunogold-labelling of thin sections demonstrated the presence of NifH (Fe protein of nitrogenase) in vegetative cells and heterocysts within five minutes of the rehydration of dried colonies. Immunogold label accumulated in discrete areas vegetative cells within 5 minutes of the rewetting of cells, and after 30 minutes a conspicuous association of NifH protein with heterocyst ribosomes was detected. After longer periods of rehydration, the deposition of gold particles became more random within both cell types but occurred with a greater frequency in heterocysts. Up to 24 hours after the rewetting of cells, two morphologically-distinct forms of heterocyst could be discerned. NifH protein was detected through Western blotting (subunit Mr=33,800) in protein extracts from samples ofNostoc commune, collected in different parts of the world and including some which had been desiccated for periods of up to 10 years. The results are discussed in relation to the sequential recovery of metabolic functions, particularly nitrogen fixation, which occurs upon the rehydration of cells after their prolonged storage in the air-dry state.
    Type of Medium: Electronic Resource
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