ISSN:
1432-1432
Keywords:
Pseudogenes
;
Amplification
;
Retroposition
;
Chromatin structure
;
DNA methylation
;
Transcription
;
RNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Although only one gene is known to be functional, numerous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) related sequences are scattered throughoutMus musculus andRattus rattus genomes. In this report we show that: (1) GAPDH pseudogenes are repeated to comparable extents, at least 400 copies, in 12 other Muridae species; (2) the complete, or nearly so, sequence of GAPDH messenger RNA is amplified, and a high proportion, if not all of these copies, are intronless; (3) GAPDH pseudogenes are preferentially located in heavily methylated and DNAse I-insensitive regions of chromatin; and (4) the presence of atypical GAPDH-related mRNAs in different cellular contexts raises the possibility that more than one GAPDH gene is transcribed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02100205
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