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  • AUXILIARY SYSTEMS  (2)
  • MATERIALS  (1)
  • PHYSICS, GENERAL  (1)
  • Phosphorylation  (1)
  • Protoplast transformation  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Purification of macrolide 2′-phosphotransferase fromStreptomyces coelicolor Müller (1990)
    Springer
    Journal of industrial microbiology and biotechnology 6 (1990), S. 295-297 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Phosphorylation ; Oleandomycin ; Macrolide 2′-phosphotransferase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary An enzyme that catalyzes 2′-O-phosphorylation of oleandomycin and several other macrolide antibiotics has been purified approximately 47-fold from cell-free extracts ofStreptomyces coelicolor Müller, NRRL 3532 (UC™ 5240). The reaction product was verified as being oleandomycin-2′-O-phosphate by mass spectrometry. As a result of purification, the enzyme was separated from two lincosaminide inactivating enzyme activities also present in the cell-free extract.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01575877
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Homologous recombination between plasmid DNA molecules in maize protoplasts (1991)
    Springer
    Molecular genetics and genomics 230 (1991), S. 209-218 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Homologous recombination ; Protoplast transformation ; β-Glucuronidase ; Maize
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The requirements for homologous recombination between plasmid DNA molecules have been studied using the PEG (polyethylene glycol)-mediated transformation system of maize (Zea mays L.) protoplasts coupled with the transient expression assay for β-glucuronidase (GUS). Two plasmids were introduced into maize protoplasts; one plasmid (pB×26) contained a genomic clone of the Adh1 maize gene; the other plasmid (piGUS) was a promoterless construction containing part of intron A of the Adhl gene fused to the gusA coding sequence. Thus, the two vectors shared an effective homologous region consisting of a 459 by (Hindlll—PvuII) fragment of the yAdh1 intron A sequence. An active gusA fusion gene would result upon homologous recombination between the plasmids within the intron A sequence, and indeed GUS activity was observed in extracts following co-transformation of maize protoplasts with the two plasmids. The presence of recombinant DNA molecules in protoplast DNA isolated 1 day after co-transformation was verified using polymerase chain reactions (PCR) and Southern blots. For efficient homologous recombination, both plasmids had to be linearized. The recombination reaction was induced by restriction of the plasmid molecules either inside the effective homologous region or at the borders of the intron sequence. However, the presence of even small, terminal, nonhomologous sequences at the 3′ end of the pB×26 fragment inhibited the recombination reaction. Also, both ends of the linearized piGUS DNA molecules were involved in the recombination reaction. The results revealed some features of homologous recombination reactions occurring in plant cells which cannot be accommodated by mechanisms postulated for similar reactions in animal system and in lower eukaryotes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00290670
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 3
    facet.materialart.
    Unbekannt
    Simplified rapid opening mechanical gate valve. (1966)
    In:  Other Sources
    Details
    Publikationsdatum: 2011-08-11
    Beschreibung: Rapid opening mechanical gate valve consisting of blade assembly, blade catcher and primer holder used in conjunction with hypervelocity free flight range experiments
    Schlagwort(e): AUXILIARY SYSTEMS
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    NASA TECHNICAL REPORTS
  • 4
    facet.materialart.
    Unbekannt
    A review of cryogenic testing performed by the thermochemical test branch, Manned Spacecraft Center in support of Apollo 13 and14 (1971)
    In:  CASI
    Details
    Publikationsdatum: 2016-06-07
    Beschreibung: The Apollo 13 anomaly provided considerable impetus for a variety of types of cryogenic and ignition tests. The logic of the various test program designs, the test techniques, and their final impact upon the investigation findings are described. In addition, several test programs initiated to determine the thermal performance and general performance characteristics of the redesigned Apollo 14 cryogenic storage system are presented.
    Schlagwort(e): PHYSICS, GENERAL
    Materialart: MSC Cryog. Symp. Papers; p 433-454
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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    NASA TECHNICAL REPORTS
  • 5
    facet.materialart.
    Unbekannt
    Evaluation of thermal insulation materials (1973)
    In:  CASI
    Details
    Publikationsdatum: 2019-06-27
    Beschreibung: Data was obtained on silicone-bonded fiberglass, isocyanurate foam, and two dozen other insulators. Materials were selected to withstand heat sterilization, outer space, and the Martian atmosphere. Significant environmental parameters were vibration, landing shock, and launch venting.
    Schlagwort(e): MATERIALS
    Materialart: NPO-11586
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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    NASA TECHNICAL REPORTS
  • 6
    facet.materialart.
    Unbekannt
    Evaluation testing of a closed Brayton-cycle electrical-power-conversion system. (1972)
    In:  Other Sources
    Details
    Publikationsdatum: 2019-07-13
    Beschreibung: Description of the design and testing of a recuperated, closed Brayton-cycle, electrical power conversion system designated the Brayton Cycle Demonstrator (BCD). The system uses electrical heaters as a heat source, argon as the cycle working fluid, and gas-lubricated foil-type bearings. Objectives of the test program include (1) evaluation of the overall system performance characteristics and influences on spacecraft integration, (2) familiarization of personnel with operational methods, and (3) determination of system flexibility by operating at a number of off-design conditions. Results obtained to date are discussed.
    Schlagwort(e): AUXILIARY SYSTEMS
    Materialart: Intersociety Energy Conversion Engineering Conference; Energy 70; Sep 21, 1970 - Sep 25, 1970; Las Vegas, NV
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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    NASA TECHNICAL REPORTS
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