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  • Protoplast  (2)
  • Boundary definition  (1)
  • Springer  (3)
  • Wiley
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Environmental management 10 (1986), S. 125-134 
    ISSN: 1432-1009
    Keywords: Wetlands ; Environmental characteristics ; Boundary definition ; Zonal properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Wetland environmental characteristics are examined to determine their spatial and temporal relationships. Two very different Oregon freshwater wetlands provided a range of wetland types. Results are evaluated to determine the possible use of environmental characteristics in defining wetlands and their boundaries. Representative physical, hydrological, and edaphic properties were periodically measured in microplots along upland/wetland transects. A multivariate approach is stressed in the data analysis; correlation, cluster analysis, and principal components analyses were used. The results indicate the environmental characteristics change in a quantifiable manner both spatially and temporally. The controlling mechanism is moisture, spatially in terms of the upland/wetland transect and temporally with respect to seasonal response. These changes do not correlate well with vegetation. Several hypotheses are offered as an explanation. Correlation within environmental characteristics is variable but definite patterns are discernible. These data suggest both single and combinations of environmental characteristics that could serve as “keys” in wetland identification and boundary determination. However, before extensive use is made of this information additional long-term monitoring of wetland environmental characteristics will be required.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 120 (1984), S. 209-215 
    ISSN: 1615-6102
    Keywords: Fusion ; Calcium ; Protoplast ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the released protoplasts to have a high potential to fuse. Protoplast fusion is enhanced by calcium and inhibited by EGTA. When 10mM calcium (pH6.0) is added, fusion percentages of 60% are common. The mild fusion treatment appears to have no effect on callus regeneration and differentiation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 124 (1985), S. 65-70 
    ISSN: 1615-6102
    Keywords: Protoplast ; Fusion ; Fluorescence ; PEG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two fluorescent compounds, scopoletin and carboxyfluorescein, have been used to label both tissue culture and leaf mesophyll cells and protoplasts. The compounds localized within the vacuoles of cells in approximately 15 hours. They remained in the vacuole during cell wall digestion, and fluorescence was observable for several hours after protoplast release. A one day pulse of these fluorescent labels had no deleterious effect on the growth of cells or protoplasts. When morphologically indistinguishable protoplasts were labeled and treated with polyethylene glycol, multicolored fluorescent fusion products were observable. These fluorescent labels provide a convenient method for selection of heterokaryon fusion products of whole plant and tissue culture cell protoplasts.
    Type of Medium: Electronic Resource
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