ISSN:
1432-203X
Keywords:
Key words Protoplasts
;
Fern gametophytes
;
Prothallial protoplasts
;
Gravity
;
Light gradients
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 m sucrose, followed by purification of the released protoplasts by floating them up into a 0.5 m sorbitol layer. Regeneration occurred over a period of 10–24 days, and, under optimal osmotic conditions, followed the developmental pattern seen during spore germination, in that the first division gave rise to a primary rhizoid. Thus, prothallial protoplasts are comparable to germinating spores as suitable models for studies of developmental polarity in single cells. As in germinating spores, the polarity of development in regenerating protoplasts is influenced by the vectors of gravity and unilateral light. However, the relative influence of light in fixing this polarity is greater in regenerating protoplasts, while in germinating spores, the influence of gravity is greater.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050470
Permalink
