ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Chiral separations
;
Human serum albumin on silica
;
Protein binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A new HPLC stationary phase was synthesized by thein situ covalent immobilization of human serum albumin (HSA). The protein was immobilized on a commerically available diol column which had been activated with 1,1-carbonyldiimidazole. Initial chromatographic studies show that this phase can be used for chiral separations of enantiomeric solutes and that these separations may reflectin vitro binding to the HSA. The effects of mobile phase composition and temperature on the stereochemical resolutions are reported.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268706
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