Publication Date:
2004-02-14
Description:
The structure of the general transcription factor IIB (TFIIB) in a complex with RNA polymerase II reveals three features crucial for transcription initiation: an N-terminal zinc ribbon domain of TFIIB that contacts the "dock" domain of the polymerase, near the path of RNA exit from a transcribing enzyme; a "finger" domain of TFIIB that is inserted into the polymerase active center; and a C-terminal domain, whose interaction with both the polymerase and with a TATA box-binding protein (TBP)-promoter DNA complex orients the DNA for unwinding and transcription. TFIIB stabilizes an early initiation complex, containing an incomplete RNA-DNA hybrid region. It may interact with the template strand, which sets the location of the transcription start site, and may interfere with RNA exit, which leads to abortive initiation or promoter escape. The trajectory of promoter DNA determined by the C-terminal domain of TFIIB traverses sites of interaction with TFIIE, TFIIF, and TFIIH, serving to define their roles in the transcription initiation process.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bushnell, David A -- Westover, Kenneth D -- Davis, Ralph E -- Kornberg, Roger D -- AI21144/AI/NIAID NIH HHS/ -- GM49985/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Feb 13;303(5660):983-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305-5126, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14963322" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Binding Sites
;
Crystallization
;
Crystallography, X-Ray
;
DNA/chemistry/metabolism
;
Models, Molecular
;
Molecular Sequence Data
;
Nuclear Magnetic Resonance, Biomolecular
;
Nucleic Acid Hybridization
;
Promoter Regions, Genetic
;
Protein Conformation
;
Protein Structure, Secondary
;
Protein Structure, Tertiary
;
RNA/chemistry/metabolism
;
RNA Polymerase II/*chemistry/metabolism
;
Saccharomyces cerevisiae Proteins/chemistry/metabolism
;
TATA Box
;
TATA-Box Binding Protein/chemistry/metabolism
;
Templates, Genetic
;
Transcription Factor TFIIB/*chemistry/metabolism
;
Transcription Factors, TFII/chemistry/metabolism
;
*Transcription, Genetic
;
Zinc/chemistry
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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