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Digitale Medien

Analysis of proteins from different phase variants of the entomopathogenic bacteria Photorhabdus luminescens by two-dimensional zymography (1997)

Ong, Kevin L. ; Chang, Frank N.

Weinheim : Wiley-Blackwell

Electrophoresis 18 (1997), S. 834-839

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ISSN: 0173-0835

Schlagwort(e): Two-dimensional zymography ; Photorhabdus luminescens ; Protease ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Two-dimensional zymography which combines two-dimensional electrophoresis with zymography was used to analyze proteases and other proteins produced by different phase variants of two strains of Photorhabdus luminescens. Both the primary and secondary phases of P. luminescens strains Hp and Hm secreted proteases. The protease in P. luminescens Hp has a molecular weight (Mr) of 57 000 and an isoelectric point (pI) of 4.4 whereas that in P. luminescens Hm has an Mr of 59 000 and pI of 4.9. Several putative protease degradation products were clearly visible in the zymograms from both bacterial strains. Two-dimensional zymography also showed that several secretory proteins were present only in particular phase variants and therefore could be used as specific markers. Unexpectedly, the two-dimensional zymography revealed that a nonsecretory protease with an Mr of 47 000 and a pI of 4.0 was present in the cell extracts of all phases of both P. luminescens Hp and Hm. The application of the two-dimensional zymography for the identification of other enzymes was also discussed.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150180530

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Digitale Medien

Complete assignment of the 13C and 1H NMR spectra of thyrsiferyl acetate (1989)

Blunt, John W. ; McCombs, John D. ; Munro, Murray H. G. ; [weitere]

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 27 (1989), S. 792-795

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ISSN: 0749-1581

Schlagwort(e): Thyrsiferol ; Thyrsiferyl acetate ; NOE ; Spectral assignments ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The assignment of all reasonances in the 13C and 1H NMR spectra of thyrsiferyl acetate has been made through the application of 2D NMR experiments, together with the use of difference NOE spectroscopy.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrc.1260270812

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ESR observations of chitin (1978)

Bruscato, Frank N. ; Holdiness, Mack ; Danti, August G.

New York : Wiley-Blackwell

Journal of Polymer Science: Polymer Chemistry Edition 16 (1978), S. 1163-1165

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ISSN: 0360-6376

Schlagwort(e): Physics ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Zusätzliches Material: 2 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/pol.1978.170160525

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