ISSN:
1615-6102
Keywords:
Duchenne muscular dystrophy
;
Dystrophin
;
Muscle cell differentiation
;
Muscle cell growth
;
Primary myotubes
;
Secondary myotubes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The Duchenne muscular dystrophy gene product dystrophin has been shown to be located on the inside of the plasma membrane. We investigated the developmental expression of dystrophin on rat skeletal muscle plasma membrane with the antiserum raised against a fragment of the polypeptide predicted from the human dystrophin cDNA map [Koenig et al. (1987) Cell 50: 509–517]. Plasma membrane of primary myotubes of the extensor digitorum longus (EDL) muscle was not initially stained by the antiserum; staining began at day 19 of embryonic life, and plasma membrane of all polynuclear muscle cells including secondary myotubes was uniformly stained by day 5 after birth. These immunohistochemical findings were supported by immunoblot analysis. These results indicate that plasma membrane of myotubes at their first appearance is not lined with dystrophin at the detectable level but becomes lined as their development proceeds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01403297
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