ISSN:
1432-2242
Keywords:
Solanum tuberosum
;
Potato plastome
;
Chloroplast DNA
;
DNA restriction profile
;
Restriction endonucleases
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Clone banks of PvuII, BamHI and XhoI fragments were generated of the Solanum tuberosum cv Katahdin plastome. These clone banks, in conjunction with molecular hybridization to tobacco ctDNA probes, were used to construct a physical map of potato ctDNA. The potato plastome was found to be a circular molecule of 155–156 Kbp containing two inverted repeat regions of 23–27 Kbp. The arrangement of restriction sites is very similar to that of other Solanaceae plastomes. Heterologous hybridization to known ctDNA encoded gene probes from tobacco allowed us to establish a genetic map of the potato chloroplast genome. The arrangement of these genes on the potato plastome resembles that on most higher plant ctDNAs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00303960
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