ISSN:
0021-9304
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
A new technique has been employed for producing Model 11 Biomer bladders with integrally textured blood-contacting surfaces. For the past year, we have been routinely fabricating integrally textured polymeric surfaces from negative-replica silicone molds, which are a high-fidelity facsimile of conventionally flocked bladders. The objectives of this investigation are threefold: (1) To develop a blood-contacting surface that promotes the formation of a stable pseudoneointimal lining; (2) to investigate means of accelerating replacement of initial fibrin coagulum by a collagenous lining; and (3) to characterize the resultant pseudoneointimal lining biochemically, histologically, and ultrastructurally. Systematic in vivo studies were undertaken to evaluate the new surface in terms of its ability to form a thin, stable, biologic lining. Control experiments were carried out to compare results with previous experience utilizing polyester fibril flocked surfaces. Additional studies were conducted to define the effects of cell seeding of integrally textured surfaces. A total of 16 experiments have been conducted to date; eight bladders were seeded with allogeneic fetal cells prior to implantation and eight bladders were unseeded, to serve as controls. In vivo trials of seeded surfaces have resulted in the formation of strong, cellular, and highly collagenous linings, with collagen contents varying between 31% and 65%. These linings contain viable cells and thus exhibit high cellular activity, as evidenced by postexplant cell culture techniques. This indicates that a living, viable pseudoneointima has been generated. On the other hand, control (nonseeded) linings do not produce significant amounts of collagen in their linings.
Additional Material:
15 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jbm.820150211
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