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  • Polymer and Materials Science  (3)
  • Regenerated silk fibroin  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Blend membrane of regenerated silk fibroin, poly(vinyl alcohol), and peroxidase and its application to a ferrocene-mediating hydrogen peroxide sensor (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 61 (1996), S. 641-647 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Before or after the blend membrane of regenerated silk fibroin (RSF), poly(vinyl alcohol) (PVA), and peroxidase is treated with ethanol, RSF, PVA, and peroxidase maintain their own structures. The conformational transition of RSF in the blend membrane is accomplished from the silk I structure to the silk II structure by ethanol treatment, which is used to immobilize peroxidase. A ferrocene-mediating sensor for H2O2 is made, which is based on the immobilization of peroxidase in the blend membrane of RSF and PVA. Performance and characteristics of the sensor were evaluated with respect to response time, detection limit, selectivity, and dependencies on temperature and pH as well as on operating and storage stability. © 1996 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Characterization of regenerated silk fibroin membranes for immobilizing glucose oxidase and construction of a tetrathiafulvalene-mediating glucose sensor (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 58 (1995), S. 973-980 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The IR spectra, the electronic absorption bands, and scanning electron microscopy (SEM) of the blend membranes of regenerated silk fibroin and glucose oxidase were reported for the first time and the second generation of glucose sensor based on glucose oxidase immobilized in the regenerated silk fibroin membrane was first constructed. The IR absorption spectra of the pure regenerated silk fibroin membrane were ascribed to its structural characteristics by which the part transition from silk I to silk II was recognized in ethanol immersion. Those spectra of the blend membrane of regenerated silk fibroin and glucose oxidase were identified as a composite of the absorption bands characteristic of both macromolecules. The electronic absorption bands showed that the glucose oxidase in the membrane exists in aggregates. A sea islands' structure was observed by SEM. These findings suggest that the regenerated silk fibroin and glucose oxidase are incompatible and their molecular interactions are very weak. A tetrathiafulvalene-mediating glucose sensor, employing immobilization of glucose oxidase by regenerated silk fibroin, was fabricated. The influences of temperature, applied potential, and pH on steady-state electrocatalytic oxidation of glucose at the sensor were evaluated. The response of the sensor to glucose under N2 saturation reached 95% steady-state current within 40 s. The sensor could be used repeatedly for 1.5 months without deterioration of the response. © 1995 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070580603
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of blend membranes of regenerated silk fibroin and PVA for immobilizing glucose oxidase and its application to a first generation of glucose sensors (1996)
    Weinheim : Wiley-Blackwell
    Electroanalysis 8 (1996), S. 480-484 
    Details
    ISSN: 1040-0397
    Keywords: Regenerated silk fibroin ; PVA ; Sensor ; Glucose oxidase ; Glucose ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The structure, water absorbability and mechanical properties of the blend membranes of regenerated silk fibroin and poly(vinyl alcohol) were investigated. IR spectra showed regenerated silk fibroin in the blend membranes exists chiefly in the silk I structure after ethanol treatment. An amperometric biosensor for glucose was constructed by coupling glucose oxidase in the blend membrane of regenerated silk fibroin and PVA with platinum electrode. Its experimental parameters such as pH, temperature and applied potential were optimized. It determined glucose in less than 25 s with good reproducibility and exhibited high storage and operational stability.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140080515
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