ISSN:
1432-0983
Keywords:
Key wordsWangiella
;
Exophiala
;
Transformation
;
Gene expression
;
Polyketide synthase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract To explore potential virulence factors in the dematiaceous (melanized) fungus Wangiella dermatitidis, we established a gene expression system with properties of homologous transformation and color identification. Using a polyketide synthase gene (WdPKS1) fragment for targeting, we found that 52% of transformants became albinos easily distinguishable from nonspecific transformants. Southern analysis confirmed that the integrations were at the WdPKS1 locus, which however did not affect transformant growth. With a heterologous promoter, P-glaA, enhanced expression of lacZ was found at 37 °C. Our results indicated that this system allows the efficient production of isogenic strains for gene function analysis in W. dermatitidis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050496
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