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  • 1
    ISSN: 1432-2048
    Keywords: Abscission (leaf) ; Leaf (abscission) ; Lyco persicon (abscission) ; Polygalacturonase ; Transgenic tomato (antisense RNA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polygalacturonase (PG, EC 3.2.1.15), an enzyme commonly found in ripening fruit, has also been shown to be associated with abscission. A zone-specific rise in PG activity accompanies the abscission of both leaves and flowers of tomato (Lycopersicon esculentum Mill.) plants. Studies of transgenic plants expressing an antisense RNA for fruit PG indicate that although the enzyme activity in transgenic fruit is 〈 1 % of that in untransformed fruit, the PG activity in the leaf abscission zone increases during separation to a similar value to that in untransformed plants. The timing and rate of leaf abscission in transgenic plants are unaffected by the introduction of the antisense gene. A polyclonal antibody raised against tomato fruit PG does not recognise the leaf abscission protein. Furthermore a complementary DNA (cDNA) clone (pTOM6), which has been demonstrated to code for fruit PG, does not hybridise to mRNA isolated from the abscission-zone region of tomato leaves. These results indicate that the PG protein in abscission zones of tomato is different from that in the fruit, and that the gene coding for this protein may also be different.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 2 (1956), S. 518-524 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper reports a direct experimental comparison of the cracking of cumene in a fluidized bed of silica-alumina catalyst with the same reaction in a fixed bed. The effects of fluidization on the kinetics of this reaction are interpreted in terms of an empirical approach using effectiveness factors and by a simplified mathematical model.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 18 (1972), S. 984-989 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A Markov chain model was used to model the axial mixing of solid particles in a motionless mixer having no moving parts. One step transition probabilities were determined experimentally for the model. Based on these transition probabilities, the model was able to predict spatial distribution of tracer particles up to seven steps of the Markov chain, which was equivalent to seven consecutive passes of the mixture through the mixer. Experimental results were in good agreement with those predicted from the Markov chain model.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 11 (1965), S. 1051-1057 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conventional data generation and analysis procedures used to select a descriptive Hougen-Watson reaction rate model have been reviewed and some statistical objections to these conventional procedures have been presented. In an attempt to assess the practical importance of these objections, a recently published example was analyzed with both conventional techniques and nonlinear least squares procedures, which more closely conform to theoretical weighting requirements. Comparisons were made between the results of the conventional linear least squares analysis of isothermal data and those of nonlinear least squares analyses of both isothermal and nonisothermal data. It was found that the nonlinear least squares procedures were useful for a rational selection of an acceptable model and estimation of its parameters.General observations were made concerning good regions for further experimentation if a discrimination among rival models is desired. Additional well-designed experiments were found to be necessary to allow a reduction of the confidence region of the parameters of the Hougen-Watson models to an acceptable size.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 2 (1956), S. 18-25 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of estimating the true conditions of operation of a bubble-cap tray is presented. Intermediate between the Murphree and the Lewis methods, which represent the extremes of actual operation, this method involves the use of a correlation to determine the degree of liquid mixing on the tray and the use of new relations between the Murphree vapor efficiency, the Lewis case I efficiency, and the true local efficiency. For the last, partial liquid mixing is taken into account.Data were obtained on an 18-in. O.D. three-tray bubble-cap tower containing ten 3-in. bubble caps a tray. Partial liquid mixing was correlated for changes in vapor and liquid rates, pressure, temperature, and weir height for the system ethylene dichloride-toluene.Efficiency data on acetone-water, ethanol-water, and ethylene dichloride-toluene showed the following effects: (1) low concentration of lwo boiler usually, but not always, resulted in low true local efficiencies, always with high Murphree efficiencies; (2) vapor velocity effects are more intimately connected with slot velocity than superficial velocity (and hence entrainment); (3) raising the pressure gives higher efficiencies; (4) an increase in liquid depth increases the true local efficiency but may have no effect on the Murphree efficiency.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 12 (1966), S. 5-10 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is reviewed which allows data points to be chosen in such a fashion that precise estimates of the parameters in nonlinear reaction rate models can be obtained. This method allows each future data point to be selected such that the confidence region of the estimated parameters is smaller with it than with any other possible data point within the region of experimentation. This procedure is applied for Hougen-Watson models with hypothetical experimental data which were generated with the guidance of an example from the current chemical engineering literature. It is found that, for the same number of data points, the parameters in the model can be estimated eighteen times more precisely by using this suggested experimental design than by another commonly used design. Confidence regions are presented for the parameters of the Hougen-Watson models with two types of designs.It is found that the positions of the data points in the well-designed experiments are more sensitive to the functional form of the model than to the current estimates of the magnitudes of the parameter values.
    Additional Material: 7 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 15 (1969), S. 320-320 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 5 (1991), S. 249-251 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A continuous-flow sample interface for fast-atom bombardment has been developed for use on an external-ion-source Fourier transform ion-cyclotron resonance mass specstrometer. The new interface provides a quick and convenient means for sample introduction. A suppression of common glycerol matrix ions was observed when compared to static analysis. High mass resolution, exact mass measurements, and tandem mass spectrometry spectra were obtained on a sample of Gramicidin S.
    Additional Material: 6 Ill.
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  • 9
    ISSN: 1617-4623
    Keywords: Down regulation ; Gene expression ; Polygalacturonase ; Sense gene ; Transgenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tomato plants were transformed with a chimaeric polygalacturonase (PG) gene, designed to produce a truncated PG transcript constitutively. In these plants expression of the endogenous PG gene was inhibited during ripening, resulting in a substantial reduction in PG mRNA and enzyme accumulation. This inhibition was comparable to that achieved previously using antisense genes. The expression of the truncated gene in ripe fruit was substantially lower than its expression in green fruit. Thus expression of both the endogenous and truncated genes is reduced in ripe fruit in which both are active. The implication of this observation is discussed in relation to the possible mechanism whereby sense constructs inhibit gene expression.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 27 (1992), S. 843-846 
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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