ISSN:
1432-2145
Schlagwort(e):
Trinucleate pollen
;
In vitro germination
;
Polyethylene glycol
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary In Brewbaker and Kwack's medium (BK) only 16% of the pollen grains germinated, and these produced pollen tubes having a maximum length of 25 μm. With a solution based on Monnier's medium 47% germination and 160-μmlong pollen tubes were observed. Calcium was shown to be essential for germination; the optimal concentration was 880 mg/l calcium chloride. The optimal concentrations of magnesium sulphate and boric acid were 360 and 50 mg/l, respectively. Germination at pH 4.0 but also pH 8.0 and the presence of vitamins B1 and B6 (1 mg/l each) were stimulatory. Polyethylene glycol (PEG) was superior to sucrose as an osmoticum and germination and tube length were significantly improved using PEG 4000 at a concentration of 120 g/l (0.03 M). Equimolar concentrations of PEG 400 and PEG 600 gave inferior results. Combining PEG with sucrose in the medium did not improve germination or increase tube length.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00202880
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