Publikationsdatum:
2010-06-05
Beschreibung:
Three-prime untranslated regions (3'UTRs) of metazoan messenger RNAs (mRNAs) contain numerous regulatory elements, yet remain largely uncharacterized. Using polyA capture, 3' rapid amplification of complementary DNA (cDNA) ends, full-length cDNAs, and RNA-seq, we defined approximately 26,000 distinct 3'UTRs in Caenorhabditis elegans for approximately 85% of the 18,328 experimentally supported protein-coding genes and revised approximately 40% of gene models. Alternative 3'UTR isoforms are frequent, often differentially expressed during development. Average 3'UTR length decreases with animal age. Surprisingly, no polyadenylation signal (PAS) was detected for 13% of polyadenylation sites, predominantly among shorter alternative isoforms. Trans-spliced (versus non-trans-spliced) mRNAs possess longer 3'UTRs and frequently contain no PAS or variant PAS. We identified conserved 3'UTR motifs, isoform-specific predicted microRNA target sites, and polyadenylation of most histone genes. Our data reveal a rich complexity of 3'UTRs, both genome-wide and throughout development.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142571/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142571/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mangone, Marco -- Manoharan, Arun Prasad -- Thierry-Mieg, Danielle -- Thierry-Mieg, Jean -- Han, Ting -- Mackowiak, Sebastian D -- Mis, Emily -- Zegar, Charles -- Gutwein, Michelle R -- Khivansara, Vishal -- Attie, Oliver -- Chen, Kevin -- Salehi-Ashtiani, Kourosh -- Vidal, Marc -- Harkins, Timothy T -- Bouffard, Pascal -- Suzuki, Yutaka -- Sugano, Sumio -- Kohara, Yuji -- Rajewsky, Nikolaus -- Piano, Fabio -- Gunsalus, Kristin C -- Kim, John K -- R00HG004515/HG/NHGRI NIH HHS/ -- R01 GM088565/GM/NIGMS NIH HHS/ -- R01 GM088565-03/GM/NIGMS NIH HHS/ -- R01GM088565/GM/NIGMS NIH HHS/ -- U01-HG004276/HG/NHGRI NIH HHS/ -- Intramural NIH HHS/ -- New York, N.Y. -- Science. 2010 Jul 23;329(5990):432-5. doi: 10.1126/science.1191244. Epub 2010 Jun 3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Genomics and Systems Biology, Department of Biology, New York University, 1009 Silver Center, New York, NY 10003, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20522740" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
*3' Untranslated Regions
;
Animals
;
Binding Sites
;
Caenorhabditis elegans/embryology/*genetics/growth & development
;
Computational Biology
;
Conserved Sequence
;
Disorders of Sex Development
;
Gene Expression Regulation, Developmental
;
Gene Library
;
*Genes, Helminth
;
Helminth Proteins/genetics
;
Histones/genetics
;
Male
;
MicroRNAs/metabolism
;
Operon
;
Poly A/metabolism
;
Polyadenylation
;
RNA, Helminth/*genetics
;
RNA, Messenger/genetics
;
Trans-Splicing
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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