ISSN:
1432-203X
Keywords:
Transgenic Arabidopsis
;
Ploidy
;
Pollen size
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00232939
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