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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 82 (1976), S. 1170-1174 
    ISSN: 1573-8221
    Keywords: Platelets ; aggregation ; refractoriness ; ADP ; cyanide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The action of potassium cyanide on ADP-induced aggregation of intact and refractory platelets was investigated. Cyanide (5·10−4 M) had virtually no effect on the aggregation of intact cells but stimulated aggregation of refractory platelets. The stimulating effect of the inhibitor on aggregation was not connected with liberation of further quantities of ADP from the cells. Differences in the sensitivity of the aggregating power of intact and refractory cells to partial depression of metabolism is discussed in terms of the “calcium” model of autoregulation of platelet aggregation suggested previously.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 44 (1988), S. 616-618 
    ISSN: 1420-9071
    Keywords: Platelets ; acridine organge ; storage granules ; fluorescence quenching ; accumulation ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new approach to the evaluation of the uptake of fluorescent, probes by intact, cells is described. Acridine organe (AO) was used because it can be selectively accumulated by serotonin-containing granules of platelets. Analysis of the fluorescence signal allows the estimation of the relative volume of the granules and the equilibrium coefficients for AO transport across the cytoplasm and granule membranes. The following results were obtained for human and rabbit platelets: the relative volumes of the granules were 14±1% and 29±2%, the ratios of intragranular-extracellular probe concentration were 2260±382 and 30,000±5500, and the cytoplasm-extracellular medium concentration ratios were 375±60 and 225±60, respectively.
    Type of Medium: Electronic Resource
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