ISSN:
1432-203X
Keywords:
Key wordsOryza sativa
;
Embryogenic cell suspension
;
Particle bombardment
;
Single-stranded DNA
;
Plasmolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Microprojectile-mediated transient and integrative transformation frequencies in rice (Oryza sativa cv. Taipei 309) embryogenic suspension cells were studied as a function of various parameters. Mannitol at concentrations of 0.5 and 0.6 m was best for osmotic preconditioning of the cells for transient, but not for integrative transformation, for which sucrose yielded the best and most reliable results. Denaturation of the transforming plasmid DNA prior to bombardment improved transient and integrative transformation frequencies two to three fold. Delivery of double-stranded plasmids in linear form had no effect on transient transformation when compared to supercoiled plasmid DNA, but led to an overall two fold increase in integrative transformation frequency. This shows that optimized protocols for generating transgenic plants should not be based exclusively on transient gene expression assays.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050611
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