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  • Plasmid  (2)
  • Protein-Energie-Mangelernährung  (1)
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  • 1
    ISSN: 1436-6215
    Keywords: protein-energymalnutrition ; vitaminE status ; children ; Sudan ; developingcountries ; Protein-Energie-Mangelernährung ; Vitamin-E-Status ; Kinder ; Sudan ; Entwicklungsländer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Das Gesamttocopherol und alpha-Tocopherol wurden im Plasma von Kindern mit schwerer Mangelernährung (14 Maramus; 11 marantischer kwashiorkor; 5 kwashiorkor) bestimmt und auf die Gesamtlipide des Plasmas und auf die verschiedenen Plasmalipidklassen bezogen. Wenn die reinen Plasmakonzentrationen als ein Index des Vitamin-E-Status genommen würden, so wären 5 Kinder mit Marasmus, 5 Kinder mit marantischem kwashiorkor und 2 Kinder mit kwashiorkor als defizitär (〈500 μg/dl) anzusehen. Werden jedoch Gesamttocopherol und alpha-Tocopherol auf die Gesamtlipide bezogen, so zeigten alle mangelernährten Kinder — bis auf eines — Werte, die innerhalb der Grenzen lagen, die für gesunde amerikanische Kinder gefunden wurden. Die Studie zeigt, daß niedrige Tocopherol-Lipid-Verhältnisse kein konstantes Merkmal bei schwer mangelernährten Kindern sind.
    Notes: Summary Total tocopherols and alpha-tocopherols were estimated in the plasma of children with severe malnutrition (14 marasmus; 11 marasmic kwashiorkor; five kwashiorkor) and related to the total plasma lipids and different plasma lipid classes. If the mere plasma concentrations were taken as an index of the vitamin E status, five children with marasmus, five children with marasmic kwashiorkor, and two children with kwashiorkor would have been regarded as deficient (〈500 μg/dl). However, if total tocopherols and alpha-tocopherols were related to the total plasma lipids, all malnourished children — except one — showed values within the limits found in healthy American children. The study shows that low tocopherol/lipid ratios are not a constant feature in severely malnourished children.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 203 (1986), S. 143-149 
    ISSN: 1617-4623
    Keywords: Plasmid ; pBR322 ; Copy number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The concentration of plasmid pBR322, of its replication inhibitor, RNAI, and preprimer, RNAII, were observed in E. coli as functions of the bacterial growth rate. At growth rates between 0.6 and 2.5 doubling/h, the copy number (number of plasmids per genome equivalent of chromosomal DNA) decreased from 32 to 15, the number of plasmids per cell increased fro, 39 to 55, and the plasmid concentration decreased from 4.6 to 1.1x1010 plasmids per OD460 unit of cell mass. The concentrations of RNAI and RNAII also decreased with incrasing growth rate, but differently, such that their ratio, RNAI/RNAII, increased. In glycerol minimal medium both RNAI and RNAII had the same halflife, 0.55 min, and were synthesized at a ratio of about 3 RNAI transcripts per every RNAII transcript. These results were interpreted on the basis of the negative control model and suggest that the activities of the RNAI and RNAII promoters, and the efficiency with which RNAI inhibits plasmid replication, are controlled by the growth rate.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 203 (1986), S. 150-153 
    ISSN: 1617-4623
    Keywords: Plasmid ; pBR322 ; relA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Replication of the plasmid pBR322, and the accumulation and life time of its primer transcript, RNAII, and replication inhibitor, RNAI, were measured in an isogenic relA + /relA pair of E. coli strains during exponential growth, or following amino acid starvation, or during treatment with chloramphenicol. (1) The synthesis rates of RNAI and RNAII decreased during inhibition of protein synthesis in either strain, i.e. their promoters are not under stringent control; (2) during amino acid starvation, RNAI and RNAII lifetimes increased in complex, rel-dependent patterns; (3) the changes in RNAI and RNAII synthesis and accumulation had no immediate effect on the rate of plasmid replication; (4) continued plasmid replication requires a protein which is synthesized during amino acid deprivation or treatment with low concentrations of chloramphenicol in relA + , but not in relA bacteria.
    Type of Medium: Electronic Resource
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