ISSN:
1432-203X
Keywords:
Key wordsHelianthus nuttallii T&G
;
Helianthus giganteus L.
;
Organogenesis
;
Plant regeneration
;
Protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Interspecific hybridisation in the genus Helianthus via somatic cell fusion is thought to play an important role in future sunflower breeding programs. The establishment of this technique requires, however, the development of single-cell-regeneration protocols. For this purpose, we applied a regeneration protocol recently developed for Helianthus annuus L. to mesophyll protoplasts of two wild sunflowers (H. nuttallii T&G, H. giganteus L). Protoplasts of both species were embedded in agarose droplets and covered by liquid mKM medium. After 4–5 weeks, callus was transferred onto solid differentiation medium yielding plating efficencies of 1.5% (H. nuttallii) and 2.5% (H. giganteus). Emerging shoots were elongated on hormone-free medium, and root formation was induced by an NAA treatment. Regenerated plants were transferred to the greenhouse where they grew up to a height of 2 m and flowered after 3 months. Seeds were harvested from regenerated plants of both species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050573
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