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  • Picloram  (2)
  • Regeneration  (1)
  • 1995-1999  (2)
  • 1910-1914
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  • 1995-1999  (2)
  • 1910-1914
Year
  • 1
    Electronic Resource
    Electronic Resource
    Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets (1998)
    Springer
    Plant cell reports 17 (1998), S. 726-730 
    Details
    ISSN: 1432-203X
    Keywords: Key words Garlic ; Allium sativum L. ; Root segments ; Regeneration ; Picloram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 4,5 μm 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μm 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μm 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050473
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  • 2
    Electronic Resource
    Electronic Resource
    Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time (1999)
    Springer
    Plant cell reports 19 (1999), S. 32-36 
    Details
    ISSN: 1432-203X
    Keywords: Key words Allium sativum ; Benzyladenine ; Picloram ; Thidiazuron ; 2 ; 4-D ; 2iP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the same basal medium with 4.7 μM picloram+0.49 μM 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 μM picloram+13.3 μM BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050706
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    Paper (German National Licenses)
    Fulltext
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