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Characterization of phospholipase A2 activation by plasmin in cultured bovine endothelial cells (1996)

Chang, Wen-Chang ; Shyu, Wei-Juin ; Shi, Guey-Yueh ; [et al.]

Springer

Journal of biomedical science 3 (1996), S. 59-66

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ISSN: 1423-0127

Keywords: Plasmin ; Calcium influx ; Cytosolic phospholipase A2 ; Endothelial cells

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Treatment of cultured bovine carotid artery endothelial cells with 0.1 µM human plasmin has been reported to induce a receptor-mediated short burst of arachidonate release, which is a pertussis toxin-sensitive and extracellular calcium-dependent reaction. Plasmin-induced calcium influx in cells was significantly inhibited by pretreatment with pertussis toxin, indicating that the former was coupled with a pertussis toxin-sensitive guanosine 5′-triphosphate (GTP)-binding protein. Plasmin significantly induced the formation of lysophosphatidylcholine but not lysophosphatidylethanolamine. A cellular phospholipase A2 with an arachidonyl specificity at the sn-2 position of phosphatidylcholine, which required submicromolar calcium, was identified as a cytosolic phospholipase A2 by immunoblot analysis. By a cell-free enzyme activity assay and immunoblot analysis, plasmin was found to induce a translocation of the cytosolic phospholipase A2 from the cytosol to the membrane. Taken together, the results suggest that plasmin bound to its putative receptor and activated a GTP-binding protein coupled to calcium influx channel, followed by translocation and activation of cytosolic phospholipase A2 in endothelial cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02253580

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New treatment of the theory for small-angle x-ray scattering with applications to polystyrene crazes (1984)

Tang, Ming-Ya ; Fellers, John F. ; Lin, J. S.

New York : Wiley-Blackwell

Journal of Polymer Science: Polymer Physics Edition 22 (1984), S. 2215-2241

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ISSN: 0098-1273

Keywords: Physics ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: The phenomenon of surface scattering of electromagnetic waves by single or multiple layers of films is reviewed and a special treatment for the total reflection of x rays is developed. This theory is applied to the analysis of the surface scattering observed in small-angle x-ray scattering (SAXS) studies of two-phase matter in polymers having lamella stacks or a flat interfacial boundary structure. Important features of this vector theory are the ability to calculate the surface scattering invariant, the absolute scattering intensity, and the surface roughness, which gives rise to dispersion of specular reflection from perfectly smooth surfaces. By considering the interfacial surface roughness of polystyrene crazes, the surface scattering spectrum is calculated theoretically and compared with some experimental results. Also the theory is presented in such a way as to compare surface scattering with volume scattering; i.e., both two- and three-dimensional scattering events can be simultaneously treated. This provides a new basis for quantitative analysis of crazes in polystyrene.

Additional Material: 20 Ill.