ISSN:
1432-2048
Keywords:
Chalcone synthase
;
Farina
;
Flavonoid biosynthesis
;
Gland
;
Phenylalanine ammonia-lyase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) were localized by indirect immunofluorescence and immunogold labeling in glands of Primula kewensis. Both enzymes were exclusively present in the head cells of the glands. Phenylalanine ammonialyase was located in the regions of the dense tubular endoplasmic reticulum and occasionally found in more or less spherical organelles that have not yet been identified. Furthermore, an appreciable proportion of the enzyme protein was associated with the plasmalemma and the cell wall of the head cell. In contrast, the occurrence of CHS was restricted to the spherical, unidentified cell compartments. Our findings indicate that the gland cells have the potential for flavonoid biosynthesis. When a mutant of P. kewensis forming structurally intact glands but incapable of farina excretion was studied, neither PAL nor CHS were found in the head cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197336
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