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  • Method of N application  (1)
  • Phenylalanine ammonia-lyase  (1)
  • 1
    ISSN: 1432-2048
    Keywords: Arachis ; Cell culture ; Enzyme induction (cell culture) ; Phenylalanine ammonia-lyase ; Phytoalexin ; Stilbene synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A peanut (Arachis hypogaea L.) cell-suspension culture susceptible to selective induction of stilbene formation was established. The principles of defense responses of the whole plant were found to be retained in the artificial system. The suspension culture was characterized by its growth curve and by various biochemical parameters. In the stationary phase, reached 8 d after transfer to a new medium, the formation of stilbenes and stilbene synthase could be induced without altering the levels of other enzymes. Eighteen hours after applying an artificial elicitor (ultraviolet-C light) or 4 h after eliciting with a crude preparation of Phytophthora cambivora cell walls, phenylalanineammonia-lyase activity was increased eightfold and stilbene-synthase activity 20-fold. The activity of phenylalanine ammonia-lyase reached its peak at a slightly different time from that of stilbene synthase. The main products of L-phenylalanine metabolism in the induced cells were resveratrol, 3,3′,5-trihydroxy-4′-methoxystilbene and isopentenylresveratrol. Likewise, feruloyl-CoA reductase, as a parameter of lignin formation, was enhanced following induction, albeit with a different time course and with a less steep increase than found for phenylalanine ammonia-lyase and stilbene synthase.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 86 (1985), S. 403-413 
    ISSN: 1573-5036
    Keywords: Basal fertilizer application ; Method of N application ; Critical N level ; Efficiency of fertilizer use ; N transformation ; N fertilizer source
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The importance of initial exchangeable soil NH 4 + in nitrogen nutrition and grain yield of rice was studied in a number of representative lowland rice soils in the Philippines. The initial exchangeable soil NH 4 + +fertilizer N plotted against nitrogen uptake by the crop resulted in a highly significant linear relationship (R2=0.91), suggesting that the presence of exchangeable NH 4 + in the soil at transplanting behaved like fertilizer nitrogen. The correlation between N fertilizer rate and N uptake by the rice crop was relatively poor (R2=0.73). On the other hand, relative grain yield was more closely correlated with the initial exchangeable soil NH 4 + +fertilizer N than with fertilizer nitrogen applied alone. These results indicate that the initial exchangeable NH 4 + in the soil contributed substantially to the nitrogen uptake of the crop. Critical nitrogen levels in the soil defined as the initial exchangeable soil NH 4 + +fertilizer N at which the optimum grain yield (95% of the maximum yield) is obtained, varied from 60 to 100 kg N/ha in the wet season and from 100 to 120 kg N/ha in the dry season for the different fertilizer treatments. The results further suggest that the initial exchangeable soil NH 4 + should serve as a guide in selecting an optimum nitrogen fertilizer rate for high grain yields.
    Type of Medium: Electronic Resource
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