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Cell-suspension culture of Arachis hypogaea L.: model system of specific enzyme induction in secondary metabolism (1987)

Rolfs, C. H. ; Schön, H. ; Steffens, M. ; [et al.]

Springer

Planta 172 (1987), S. 238-244

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ISSN: 1432-2048

Keywords: Arachis ; Cell culture ; Enzyme induction (cell culture) ; Phenylalanine ammonia-lyase ; Phytoalexin ; Stilbene synthase

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A peanut (Arachis hypogaea L.) cell-suspension culture susceptible to selective induction of stilbene formation was established. The principles of defense responses of the whole plant were found to be retained in the artificial system. The suspension culture was characterized by its growth curve and by various biochemical parameters. In the stationary phase, reached 8 d after transfer to a new medium, the formation of stilbenes and stilbene synthase could be induced without altering the levels of other enzymes. Eighteen hours after applying an artificial elicitor (ultraviolet-C light) or 4 h after eliciting with a crude preparation of Phytophthora cambivora cell walls, phenylalanineammonia-lyase activity was increased eightfold and stilbene-synthase activity 20-fold. The activity of phenylalanine ammonia-lyase reached its peak at a slightly different time from that of stilbene synthase. The main products of L-phenylalanine metabolism in the induced cells were resveratrol, 3,3′,5-trihydroxy-4′-methoxystilbene and isopentenylresveratrol. Likewise, feruloyl-CoA reductase, as a parameter of lignin formation, was enhanced following induction, albeit with a different time course and with a less steep increase than found for phenylalanine ammonia-lyase and stilbene synthase.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00394593

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Komplexchemisches Verhalten von funktionellen Derivaten des 2-Äthyl-pyridins. I. Das 2-(β-Hydroxyäthyl)-pyridinProfessor Franz Hein zum 70. Geburtstage am 30. Juni 1962 gewidmet (1962)

Uhlig, E. ; Schön, H.

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 316 (1962), S. 25-33

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ISSN: 0044-2313

Keywords: Chemistry ; Inorganic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: 2-(β-Hydroxyethyl)-pyridine reacts with Cu(II)-, Zn(II)-, Co(II)-, and Ni(II)ions forming 1,2-complexes. Zn(II) forms an 1,1-complex, too. No 1,3-complex has been isolated.The organic ligand is for the most part a bidentate one. CoCl2 and NiCl2, however, yield co-ordination compounds in which only the N-atom of the pyridine ring reacts as a donator.

Notes: Das 2-(β-Hydroxyäthyl)-pyridin bildet mit Kupfer(II)-, Zink(II)-, Kobalt(II)- und Nickel(II)-Ionen 1,2-Komplexe. Vom Zink(II) wird auch ein 1,1-Komplex beschrieben. In keinem Fall gelang die Isolierung von 1,3-Komplexen. Der Komplexbildner fungiert in den meisten Fällen als zweizähliger Ligand. Bei der Umsetzung mit Kobalt(II)- bzw. Nickel(II)-chlorid entstehen jedoch Komplexverbindungen, in denen nur der Pyridinstickstoff als Donator wirkt.

Additional Material: 1 Ill.