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  • 1
    Electronic Resource
    Electronic Resource
    Specialization for ureide biogenesis in the root nodules of black locust (Robinia pseudoacacia L.), an amide exporter (1990)
    Springer
    Protoplasma 157 (1990), S. 102-111 
    Details
    ISSN: 1615-6102
    Keywords: Peroxisomes ; Robinia ; Root-nodules ; Ureide biogenesis ; Uricase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Black locust (Robinia pseudoacacia L.), a member of the legume tribe Robinieae, has indeterminate root nodules and is primarily an amide exporter. However, in greenhouse-grown seedlings inoculated with rhizobia, ureide-N comprises approximately 8% of the total soluble-N in the xylem sap. Ultrastructurally, young interstitial cells (i.e., uninfected cells in the infected region near the nodule meristem) develop enlarged peroxisomes and abundant tubular ER, properties heretofore found to be characteristic only of members of the Phaseoleae, which have determinate nodules and are exporters principally of ureides. Many of the peroxisomes in the interstitial cells of black locust nodules react strongly for uricase (EC 1.7.3.3) activity in a cytochemical test employing diaminobenzidine, but some of the peroxisomes, particularly those farther back from the meristem, react weakly or not at all, even though enlarged. The ultrastructural specializations of the interstitial cells seem disproportionately large in comparison to the relatively low levels of ureides in the xylem transport stream. We suggest that similar specialization of some of the interstitial cells might be found in other legumes that export low amounts of ureides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322642
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  • 2
    Electronic Resource
    Electronic Resource
    Specialization of the inner cortex for ureide production in soybean root nodules (1989)
    Springer
    Protoplasma 150 (1989), S. 150-159 
    Details
    ISSN: 1615-6102
    Keywords: Inner cortex ; Peroxisomes ; Root-nodules ; Soybean ; Uricase cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The possibility that cells in the inner cortex of determinate root nodules participate in ureide production from recently fixed N2, as do the uninfected (interstitial) cells of the infected central region, has been investigated in soybean (Glycine max) inoculated as seeds withBradyrhizobium japonicum. Like the interstitial cells, cells of the three innermost cortical layers produce enlarged peroxisomes and a meshwork of tubular ER during differentiation. These changes are most pronounced in the innermost cortical layer, are successively less so in the 2nd and 3rd layers, and are usually undetectable in more distant layers. Peroxisomes in the inner three layers are stained in the DAB (3,3′-diaminobenzidine) test for uricase (EC 1.7.3.3) activity, indicative of the potential for ureide formation, but peroxisomes in more distant cortical cells are not stained. A nodulespecific uricase also is demonstrable in the inner three cortical layers by immunogold labeling enhanced with silver for visualization in the light microscope. The observations suggest that with respect to ureide production the cells of the inner layers of the cortex are functionally similar to the interstitial cells of the infected region despite the apparent distinctiveness of the two regions anatomically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403671
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  • 3
    Electronic Resource
    Electronic Resource
    Cytochemical localization of uricase and catalase in developing root nodules of soybean (1987)
    Springer
    Protoplasma 140 (1987), S. 1-12 
    Details
    ISSN: 1615-6102
    Keywords: Cerium chloride ; Diaminobenzidine (DAB) ; Peroxisomes ; Root-nodules ; Soybean ; Uricase cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two different cytochemical methods were used to study the localization of uricase (EC 1.7.3.3) and catalase (EC 1.11.1.6) in developing root nodules of soybean (Glycine max) inoculated as seeds withBradyrhizobium japonicum. One of the methods employs DAB (3,3′-diaminobenzidine) and detects uricase activity indirectly by coupling it to endogenous catalase activity. The other method utilizes cerium chloride to detect uricase activity directly. These methods were modified to obtain not only a strong staining reaction but also improved ultrastructural preservation. With the indirect DAB method, intense staining indicative of both uricase and catalase activity was obtained in the enlarged peroxisomes of older uninfected cells. Similar staining was observed in enlarging peroxisomes of younger uninfected cells, and in the material of associated sacs whose bounding membranes appear to arise as distensions of the ER. The observations are discussed in relation to the controversial role of the ER in peroxisome biogenesis. Although the small peroxisome-like organelles of infected cells did not give a clearly positive reaction in the indirect DAB method, they reacted positively in the cerium chloride method, and are considered to be peroxisomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273250
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