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  • 1
    Electronic Resource
    Electronic Resource
    Purification of cationic peroxidases bound ionically to the cell walls from the roots ofZinnia elegans (1995)
    Springer
    Journal of plant research 108 (1995), S. 463-468 
    Details
    ISSN: 1618-0860
    Keywords: Amino acid sequence ; Lignification ; Peroxidase isoenzymes ; Tracheary element differentiation ; Zinnia elegans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell wall-bound and tracheary element-specific peroxidase isoenzymes, designated P5A and P5B, were shown previously to be associated with lignification during the differentiation into tracheary elements of single cells isolated from the mesophyll ofZinnia elegans (Satoet al. Planta 189: 584–589, 1993; Planta 196: 141–147, 1995). Isoenzymes corresponding to P5 (RP5A and RP5B) were present at a relatively high level in the roots ofZinnia elegans. These isoenzymes were purified from theZinnia roots by several column-chromatographic steps. Both RP5A and RP5B had molecular masses of 35 kDa. Purified RP5A and RP5B were cleaved by CNBr and the partial amino acid sequences of these isoenzymes were determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344235
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  • 2
    Electronic Resource
    Electronic Resource
    Separation and characterization of the isoenzymes of wall-bound peroxidase from cultured Zinnia cells during tracheary element differentiation (1995)
    Springer
    Planta 196 (1995), S. 141-147 
    Details
    ISSN: 1432-2048
    Keywords: Cell wall ; Coniferyl alcohol ; Lignification ; Peroxidase isoenzymes ; Tracheary element differentiation ; Zinnia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell wall-bound peroxidase (EC 1.11.1.7) isoenzymes (P1-P5) from cells of Zinnia elegans L. that were differentiating into tracheary elements were separated and characterized to obtain information about the relationships between these isoenzymes and the biosynthesis of lignin. Fractionation of Zinnia cells by centrifugation in solutions of Percoll revealed that P1, P2, and P5 were present in differentiated tracheary elements. These peroxidase isoenzymes were separated by several column-chromatographic steps. During hydrophobic chromatography on Phenyl Superose, P5 activity was separated into activities P5A and P5B. Enzymatically pure preparations of P1, P3, P5A, and P5B were finally obtained and used for the characterization of each isoenzyme. The optimum pH was 5.5–6.0 for P1, 5.0–7.5 for P3, 5.0 for P5A, and 4.0 for P5B. Each of the isoenzymes oxidized coniferyl alcohol efficiently, whereas p-coumaryl alcohol and sinapyl alcohol were poor substrates for all the isoenzymes. An absolute requirement for Ca2+ ions was demonstrated for P3. Based on these results, possible roles of peroxidase isoenzymes in the formation of lignin during the differentiation of tracheary elements are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193227
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  • 3
    Electronic Resource
    Electronic Resource
    Interrelationship between lignin deposition and the activities of peroxidase isoenzymes in differentiating tracheary elements of Zinnia (1993)
    Springer
    Planta 189 (1993), S. 584-589 
    Details
    ISSN: 1432-2048
    Keywords: 2-Aminoindan-2-phosphonic acid ; l-α-Aminooxy-β-phenylpropionic acid ; Peroxidase isoenzymes ; Tracheary element differentiation ; Zinnia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a culture system in which single cells isolated from the mesophyll of Zinnia elegans L. differentiate to tracheary elements (TEs), two inhibitors of phenylalanine ammonia-lyase (EC 4.3.1.5), L-α-aminooxy-β-phenylpropionic acid (AOPP) at 10 μM inhibited lignification without reducing the number of TEs formed. These inhibitors caused intracellular changes in peroxidase (EC 1.11.1.7) activities. The inhibitors increased the activity of peroxidases bound to the cell walls and especially the activity of peroxidase bound ionically to the cell walls. In contrast, the activity of extracellular peroxidase decreased. There were five isoenzymes, P1-P5, in the ionically bound peroxidase of cultured Zinnia cells. Among the isoenzymes, P4 and P5 appeared to be specific for TE differentation. Treatment with AOPP and AIP resulted in increases in the activities of P2, P4 and P5 isoenzymes, with the most prominent increase in P5 activity. The addition of lignin precursors, including coniferyl alcohol, to the AOPP-treated cells restored lignification, and suppressed the alteration of peroxidase isoenzyme patterns caused by AOPP. The relationship between the wall-bound peroxidases and lignification during TE differentiation is discussed in the light of these results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198223
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