ISSN:
1617-4623
Keywords:
pepD gene
;
gpt gene
;
Intergenic region
;
lpcA locus
;
Peptidase D purification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A cloned DNA fragment, carrying the gene for peptidase D (pepD) of Escherichia coli, was partially sequenced. By purification of peptidase D and sequence determination of an amino-terminal oligopeptide the reading frame of the pepD gene, starting with a GTG initiator codon, was unambiguously identified. An overlap of the established nucleotide sequence with the previously sequenced 5′ flanking region of the gpt gene allowed the exact distance between pepD and gpt to be calculated. The two genes are pointing towards each other and are separated by 260 bp. A search for open reading frames (ORFs) and the analysis of possible codon usage in the intercistronic region indicate the absence of an additional gene (lpcA) between pepD and gpt.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00427031
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