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  • Articles  (2)
  • Pavlova  (1)
  • Prior self-pollination  (1)
  • 1990-1994  (2)
  • 1880-1889
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  • Articles  (2)
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  • 1990-1994  (2)
  • 1880-1889
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 7 (1994), S. 273-281 
    ISSN: 1432-2145
    Keywords: Pollen-pistil interference ; Prior self-pollination ; Tristyly ; Self-incompatibility Stylar senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The potential inhibitory effects of incompatible pollen on outcrossed seed set were investigated in mass-flowering, self-incompatible, tristylous Pontederia sagittata. Prior application of self pollen, followed after 2, 4, or 6 h by compatible pollen, was conducted on five genotypes of each of the three style morphs under uniform glasshouse conditions. The greatest reductions in seed set occurred in pollinations of the long-styled (L) morph at the 6 h time interval. Smaller reductions were also found for this treatment in the mid-styled (M) morph. No significant reductions in seed set were observed in the short-styled (S) morph or in the other morphs at shorter time intervals. Observations of pollen germination and pollen tube growth indicated that the lack of inhibitory effects in the S morph may occur because relatively few pollen grains adhered to stigmas in selfpollinations. In the L and M morphs, early germination of self pollen may cause physical clogging of the stigma and style, resulting in a reduced number of compatible pollen tubes in styles. Observations of the structural integrity of styles indicated that prior germination of self pollen resulted in more rapid onset of pistil senescence, particularly in the L morph. These influences may contribute to the morph-specific differences in seed set observed following prior self-pollination of outcrossed flowers. The negative effects of incompatible pollen are likely to be most evident where ecological factors cause delays in the delivery of outcross pollen to stigmas.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5176
    Keywords: Isochrysis ; lipid ; mariculture ; mass culture ; microalgae ; Nannochloropsis ; nutrition ; Pavlova ; polyunsaturated fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three species of microalgae were grown in mass culture to investigate the influence of culture technique and growth phase on the production of 20:5(n−3) and 22:6(n−3). These polyunsaturated fatty acids (PUFA) are considered to be essential in many marine animals diets for high growth and survival rates. The species of microalgae examined wereNannochloropsis oculata, Pavlova lutheri andIsochrysis sp. (clone T.Iso). All batch cultures (logarithmic and stationary phase) and semi-continuous cultures (logarithmic phase) examined contained high levels of the long-chain (n−3) PUFA, but production could be maximised by harvesting at specific times and growth phases. Maximum cellular content (pg cell-1) of long-chain PUFA was found in logarithmic phase batch cultures ofN. oculata and in stationary phase cultures ofP. lutheri. The cellular content of PUFA in cultures ofIsochrysis sp. did not change significantly with culture technique or growth phase. Alternatively, stationary phase cultures of all three species showed increased proportions (%) and cellular contents of triacylglycerols, and saturated and monounsaturated fatty acids with correspondingly decreased proportions of polar lipids and most PUFA relative to logarithmic phase cultures. The exception was the proportion and cellular content of 22:6(n−3) inP. lutheri which increased with triacylglycerol content. The mass of long-chain (n−3) PUFA per volume of culture was significantly higher in stationary phase cultures due to the higher cell counts per volume. These findings indicate that the opportunity exists to maximise PUFA production by microalgae with the potential to improve animal growth and reduce production costs in mariculture operations and may be of use in the large scale culture and harvesting of microalgae for the biotechnology industry.
    Type of Medium: Electronic Resource
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