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  • PACS: 41.20.Bt; 42.62.-b; 42.62.Be; 61.20.Ja; 87.80.+s; +94.30.Hn  (2)
  • Dispersion  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Force measurements of optical tweezers in electro-optical cages (1998)
    Springer
    Applied physics 67 (1998), S. 385-390 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 41.20.Bt; 42.62.-b; 42.62.Be; 61.20.Ja; 87.80.+s; +94.30.Hn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390050790
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  • 2
    Electronic Resource
    Electronic Resource
    Processing of micro-particles by UV laser irradiation in a field cage (1999)
    Springer
    Applied physics 69 (1999), S. 611-616 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 41.20.Bt; 42.62.-b; 42.62.Be; 61.20.Ja; 87.80.+s; +94.30.Hn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. An electric cage-laser micro-turning lathe was realised and applied to contact-free handling and mechanical processing of micro particles. Since particles with diameters of several micrometers cannot be fixed in mechanical chucks, an octode field cage was used to trap and rotate a single particle in a fluid without any mechanical surface contact. A pulsed nitrogen laser of high beam quality focused to about 1 μm in diameter could be adjusted independently of the cage position. The trapping forces (negative dielectrophoresis) acting on a bead of 5 to 15 μm are up to several hundred pN. This and the surrounding fluid damp down the effect of the laser pulses during bead processing. Examples demonstrating the possibilities of this technique are shown. Microsystems with high optical quality were fabricated photolithographically or by laser direct-write chemical vapor deposition (LCVD). Technical and biotechnological applications are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390051040
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  • 3
    Electronic Resource
    Electronic Resource
    Electrorotational spectra of protoplasts generated from the giant marine algaValonia utricularis (1997)
    Springer
    Protoplasma 196 (1997), S. 123-134 
    Details
    ISSN: 1615-6102
    Keywords: Valonia utricularis ; Protoplast preparation ; Electrorotation ; Microstructures ; Dispersion ; Polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts ofValonia utricularis lacking the large central vacuole can be generated by cutting multi-nucleated, giant “mother” cells into small pieces after short exposure to air. When the protoplasmic content was squeezed out into sea water, irregularly shaped, green coloured aggregates were formed which changed into spherical protoplasts (radius of 20–60 μm) after about 2 h. In these protoplasts the dense internal material (consisting mainly of organelles) was separated from the plasmalemma by a thin transparent layer containing a large number of small lipid vesicles. Cell wall regeneration occurred rapidly after protoplast formation. A central vacuole developed after about 10h. The regenerated cells continued to grow and were viable for several months. Electrorotation studies on 2–3 h old protoplasts at pH 7 in low- and fairly high-conductivity solutions showed one or two anti-field rotation peaks (depending on medium conductivity) between 10 kHz to 1 MHz as well as one cofield rotation peak between 10 MHz to 100 MHz. The rotation spectra could not be fitted on the basis of the single- (or multi-) shell model (i.e., by modelling the cells as a homogeneous sphere surrounded by one or more layers). However, fairly good agreement between the experimental data and theory could be obtained by assuming that the rotational behaviour of the protoplasts depends not only on passive electrical properties of the plasmalemma but is influenced by “mobile charges” of carrier transport systems and/or the dielectric behaviour of the aggregated chloroplasts and vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279561
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