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  • Osteoblasts  (2)
  • critical electrolyte concentration  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Paradoxical effect of 1.25 dihydroxycholecalciferol on osteoblastic and osteoclastic activity in the skeleton of the eelAnguilla anguilla L (1980)
    Springer
    Calcified tissue international 32 (1980), S. 83-87 
    Details
    ISSN: 1432-0827
    Keywords: 1.25 (OH)2D3 ; Fish ; Bone ; Osteoblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Female mature eels (300 to 500 g) received one intraperitoneal injection of 1.25 (OH)2D3 (10 µg). Their vertebral bone was compared, 8 h and 24 h after the injection, with vertebral bone of control mature female eels receiving solvent alone (ethanol). Sexual maturation in female eels induces a bone decalcification with hypercalcaemia and hyperphosphataemia. The control eels showed marked osteoclastic resorption and osteocytic osteolysis and the degree of mineralization of the intercellular substance decreased. Injection of 1.25 (OH)2D3 into these female mature eels provoked as early as 8 h : 1) an increase in hypercalcaemia and hyperphosphataemia ; 2) a major conversion of lining cells to osteoblasts and a stimulation of osteoblastic activity with new bone formation ; 3) diminished osteoclastic resorption without changing osteocytic osteolysis or bone matrix mineralization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408525
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Apoptosis: identification by a critical electrolyte concentration method (1996)
    Springer
    Apoptosis 1 (1996), S. 218-221 
    Details
    ISSN: 1573-675X
    Keywords: Apoptosis ; critical electrolyte concentration ; cytochemistry ; nucleic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A critical electrolyte concentration method previously proposed to distinguish RNA from DNA was considered for a rapid screening of apoptosis at the cellular level. For the monitoring of the apoptotic phenomenon, preparations subjected to anin situ 3′ end DNA labelling assay were also analyzed. The results indicated that the critical electrolyte concentration assay as used here is a simple and useful tool for the rapid identification of apoptotic cells and could contribute to the determination of their frequency, localization and other characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01321106
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  • 3
    Electronic Resource
    Electronic Resource
    Evidence for the presence of osseous tissue in dogfish vertebrae (1982)
    Springer
    Cell & tissue research 222 (1982), S. 605-614 
    Details
    ISSN: 1432-0878
    Keywords: Elasmobranch ; Endoskeleton ; Osseous tissue ; Osteoblasts ; Osteocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The calcified cartilage of the dogfish vertebra has been studied by means of an undecalcified hard tissue method, including microradiography and tetracycline labelling, and electron microscopy. The transversely sectioned vertebra shows a centrum and neural and hemal arches. The mineralized area consists of a narrow but continuous band, which touches the perichondrium, and is formed by chondrocytes that participate in the mineralization of the surrounding matrix. The neural arches appear quite different; the upper parts contain an hypertrophied cartilage and, close to it, an inner zone formed by crescent shaped lamellar bone tissue containing osteoblasts and osteocytes. Tetracycline labelling of these two types of hard tissue reveals a globular calcification with calcospherites and Liesegang rings, at the level of the calcified cartilage, and a strong and linear label of the inner border of the osseous tissue. Transmission electron microscopy shows Type I collagen in the crescent shape area and Type II collagen in calcified cartilage area. The presence of osseous tissue in elasmobranch endoskeleton is discussed in relation to the evolution of the gnathostomes skeleton and the endocrinological control of calcium metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213858
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