ISSN:
1432-072X
Keywords:
Creatinine degradation
;
Pseudomonas putida 77
;
Pseudomonas sp. H21
;
Pseudomonas sp. 0114
;
N-Methylhydantoin degradation
;
Creatinine deiminase
;
N-Methylhydantoin hydrolase
;
N-Carbamoylsarcosine amidohydrolase
;
Creatine amidinohydrolase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The microbial decomposition of creatinine was found to proceed mainly via N-methylhydantoin or creatine as the first degradation product. Either N-methylhydantoin or urea or both were detected as metabolites derived from creatinine in various microorganisms, and creatinine deiminase and creatinine amidohydrolase activities were detected concomitantly. N-Methylhydantoin hydrolase and N-carbamoylsarcosine amidohydrolase were found to be formed inducibly in the presence of creatinine or N-methylhydantoin. Three microorganisms which decompose creatinine in different ways were screened from soil. Pseudomonas putida 77 rapidly metabolized creatinine solely via N-methylhydantoin. Degradation of creatinine proceeded with both creatine and N-methylhydantoin as the first degradation products at the same time in Pseudomonas sp. H21. Pseudomonas sp. 0114 was found to metabolize creatinine mainly via creatine and to also metabolize N-methylhydantoin. Changes in the metabolites of creatinine during a cultivation or enzyme reaction were found to be closely related to the enzyme activities of interest which are regulated by creatinine or N-methylhydantoin in different ways depending on the microbial strain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00470865
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