ISSN:
1432-072X
Keywords:
Transfection
;
Mycobacterium smegmatis
;
Mycobacteriophage I3
;
Calcium sensitization
;
Glycine sensitization
;
Protamine sulfate
;
Exogenous RNA
;
DNA uptake
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or CaCl2, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From 32P DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of DNA which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425216
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