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  • 1
    Electronic Resource
    Electronic Resource
    An embryogenic cell line of maize from A188 (Minnesota) contains Mu1-like elements (1988)
    Springer
    Plant molecular biology 10 (1988), S. 273-279 
    Details
    ISSN: 1573-5028
    Keywords: embryogenic cell culture ; maize ; Mutator ; transposable elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize inbred line A188 is popularly used for the production of embryogenic cell lines. A188, maintained at the University of Minnesota, was found upon molecular analysis to contain 2 to 4 copies of a DNA sequence very similar in structure to transposable Mu1 elements, which have been implicated in Robertson's Mutator system. These Mu1-like elements are in the same chromosomal locations in sibling plants and in A188 cell cultures derived from them. This suggests that the elements are in an inactive state and do not undergo transposition. However, we have observed that they are not modified at the target sites for certain restriction endonucleases. Possible causes for the apparent lack of transposition of these Mu1-like elements in these A188 lines are discussed. Inasmuch as the elements do not transpose, they must be maintained in this line as homozygous Mendelian elements by self-pollination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027404
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  • 2
    Electronic Resource
    Electronic Resource
    Concomitant regulation of Mu1 transposition and Mutator activity in maize (1987)
    Springer
    Molecular genetics and genomics 208 (1987), S. 57-62 
    Details
    ISSN: 1617-4623
    Keywords: Transposable elements ; Mutation ; Mutator ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutagenic activity of the maize transposable element system Mutator can be lost by outcrossing to standard, non-Mutator lines or by repetitive intercrossing of genetically diverse Mutator lines. Lines losing Mutator mutagenic activity in either manner retain high copy numbers (10–15 per diploid genome) of the Mutator-associated Mu transposable elements. Frequent transposition of Mu1-related elements is observed only in active Mutator lines, however. The loss of Mutator activity on intercrossing is correlated with an increase in the copy number of Mu1-like elements to 40–50 per diploid genome, implying a self-encoded or self-activated negative regulator of Mu1 transposition. The outcross loss of Mutator activity is only weakly correlated with a low Mu element copy number and may be due to the loss of a positive regulatory factor encoded by a subset of Mu1-like elements. Transposition of Mu elements in active Mutator lines generates multiple new genomic positions for about half the elements each plant generation. The appearance of Mu1-like elements in these new positions is not accompanied by equally high germinal reversion frequencies, suggesting that Mu1 may commonly transpose via a DNA replicative process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330422
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