Publication Date:
2006-03-11
Description:
A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single-base pair substitutions, insertions, and deletions, and most (〉90%) of the approximately 30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single-base pair changes accumulated during experimental evolution of yeast.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gresham, David -- Ruderfer, Douglas M -- Pratt, Stephen C -- Schacherer, Joseph -- Dunham, Maitreya J -- Botstein, David -- Kruglyak, Leonid -- P50 GM071508/GM/NIGMS NIH HHS/ -- R01 GM046406/GM/NIGMS NIH HHS/ -- R37 MH059520/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2006 Mar 31;311(5769):1932-6. Epub 2006 Mar 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA. dgresham@genomics.princeton.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16527929" target="_blank"〉PubMed〈/a〉
Keywords:
Directed Molecular Evolution
;
Genes, Fungal
;
*Genome, Fungal
;
Genomics
;
Mutation
;
Nucleic Acid Hybridization
;
*Oligonucleotide Array Sequence Analysis
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Phenotype
;
Point Mutation
;
*Polymorphism, Single Nucleotide
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Saccharomyces cerevisiae/*genetics/physiology
;
Sequence Deletion
;
Suppression, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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