Publikationsdatum:
2006-01-21
Beschreibung:
Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bhattacharyya, Roby P -- Remenyi, Attila -- Good, Matthew C -- Bashor, Caleb J -- Falick, Arnold M -- Lim, Wendell A -- New York, N.Y. -- Science. 2006 Feb 10;311(5762):822-6. Epub 2006 Jan 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cellular and Molecular Pharmacology, University of California-San Francisco, 600 16th Street, San Francisco, CA 94143-2240, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16424299" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Adaptor Proteins, Signal Transducing/*chemistry/genetics/*metabolism
;
Allosteric Regulation
;
Amino Acid Motifs
;
Binding Sites
;
Crystallography, X-Ray
;
Down-Regulation
;
Enzyme Activation
;
*MAP Kinase Signaling System
;
Mitogen-Activated Protein Kinases/*chemistry/*metabolism
;
Models, Biological
;
Models, Molecular
;
Mutation
;
Pheromones/*physiology
;
Phosphorylation
;
Protein Binding
;
Protein Conformation
;
Protein Structure, Secondary
;
Protein Structure, Tertiary
;
Saccharomyces cerevisiae/genetics/*metabolism
;
Saccharomyces cerevisiae Proteins/*chemistry/genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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