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  • Embryonic development  (1)
  • Guinea pig  (1)
  • Muscle ultrastructure  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 134 (1972), S. 1-11 
    ISSN: 1432-0878
    Keywords: Muscle ultrastructure ; (Na+, K+)-ATPase localization ; Sarcotubules ; Sarcoplasmic reticulum ; Junctional SR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary ATPase activity sensitive to ouabain was examined in both cardiac (ventricular) and skeletal (tibialis anterior) muscle cells of the mouse. Short-term fixation was combined with incubation in a medium designed to reduce artifactual deposition of lead phosphate. With incubation medium containing Na+ and K+, Pb3 (PO4)2 precipitate appears throughout the sarcoplasmic reticulum (SR) of both cardiac and skeletal cells. The precipitate generally is heavier in the junctional SR than in network SR, although the two regions are interconnected. Ouabain (1 mM) eliminates activity in the network SR of myocardial cells, but only reduces it in skeletal muscle cells. The total ATPase activity of junctional cisternae of the SR of myocardial cells does not appear to be reduced by ouabain, whereas the activity of the terminal cisternae of skeletal muscle is substantially diminished. The use of an incubation medium containing zero K+ reduces the level of activity, but not consistently. These data suggest that (Na+, K+)-ATPase is present in the network SR of both cardiac and skeletal muscle cells, and probably in the terminal cisternae of skeletal muscle cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 166 (1976), S. 83-90 
    ISSN: 1432-0878
    Keywords: Embryonic development ; Ventricular myocardium ; Transverse and axial tubules ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Developing transverse (T) tubules are found in embryonic guinea pig ventricular myocardium after approximately eight weeks of gestation. By the time of birth (nine weeks total gestation), longitudinally-oriented axial tubules connected to the T tubules also have formed, and the majority of cells closely resemble those of the adult. The form taken by the developing T and axial tubules suggests that they are generated in a manner similar to that for T tubules in chick and rat skeletal muscle, namely by repeated formation of caveolae.
    Type of Medium: Electronic Resource
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