ISSN:
1432-0827
Keywords:
Vitamin D3 metabolites
;
Cellular calcium
;
Muscle
;
Myoblasts
;
Tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Summary 25-hydroxycholecalciferol (25OHD3) and 1,25-dihydroxycholecalciferol (1,25(OH)2D3) at physiological concentrations exerted direct effects on Ca fluxes in cultured vitamin D-deficient chick soleus muscle and myoblasts. Isotopic desaturation curves of soleus muscle prelabeled with45Ca indicated that the action of 25OHD3 is localized in a slow-exchangeable Ca pool where it stimulates net Ca uptake. On the other hand, the predominant effects of 1,25(OH)2D3 consist in an increase of the rate constant of Ca efflux of this pool and in an increase of net Ca uptake in a fast-exchangeable pool. 24,25-dihydroxycholecalciferal proved to be inactive on both Ca uptake and efflux. In addition, 1,25(OH)2D3 significantly increased45Ca labeling of cultured chick myoblasts. These effects were accompanied by changes in the growth and differentiation of the cultures. The results suggest a direct involvementin vivo of 25OHD3 and 1,25(OH)2D3 on muscle cellular calcium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02405318
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