Publication Date:
1987-09-18
Description:
The protein portion of the immunosuppressive glycoprotein uromodulin is identical to the Tamm-Horsfall urinary glycoprotein and is synthesized in the kidney. Evidence that the glycoproteins are the same is based on amino acid sequence identity, immunologic cross-reactivity, and tissue localization to the thick ascending limb of Henle's loop. Nucleic acid sequencing of clones for uromodulin isolated from a complementary DNA bank from human kidney predicts a protein 639 amino acids in length, including a 24--amino acid leader sequence and a cysteine-rich mature protein with eight potential glycosylation sites. Uromodulin and preparations of Tamm-Horsfall glycoprotein bind to recombinant murine interleukin-1 (rIL-1) and human rIL-1 alpha, rIL-1 beta, and recombinant tumor necrosis factor (rTNF). Uromodulin isolated from urine of pregnant women by lectin adherence is more immunosuppressive than material isolated by the original salt-precipitation protocol of Tamm and Horsfall. Immunohistologic studies demonstrate that rIL-1 and rTNF bind to the same area of the human kidney that binds to antiserum specific for uromodulin. Thus, uromodulin (Tamm-Horsfall glycoprotein) may function as a unique renal regulatory glycoprotein that specifically binds to and regulates the circulating activity of a number of potent cytokines, including IL-1 and TNF.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hession, C -- Decker, J M -- Sherblom, A P -- Kumar, S -- Yue, C C -- Mattaliano, R J -- Tizard, R -- Kawashima, E -- Schmeissner, U -- Heletky, S -- New York, N.Y. -- Science. 1987 Sep 18;237(4821):1479-84.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3498215" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Cloning, Molecular
;
Enzyme-Linked Immunosorbent Assay
;
Glycoproteins/metabolism
;
Humans
;
Interleukin-1/metabolism
;
Kidney/*metabolism
;
Ligands/metabolism
;
Lymphokines/*metabolism
;
Molecular Weight
;
Mucoproteins/*analysis/genetics
;
RNA, Messenger/analysis
;
Recombinant Proteins/metabolism
;
Tumor Necrosis Factor-alpha
;
Uromodulin
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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