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  • 1
    Electronic Resource
    Electronic Resource
    Evidence that genes from the male parent may influence the morphology of potato dihaploids (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 241-248 
    Details
    ISSN: 1432-2242
    Keywords: Key words Potato dihaploids ; Principal component analysis ; Dihaploid induction ; Microsatellite ; Isozyme analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of recent studies have provided evidence that potato dihaploids (S. tuberosum) contain and express DNA from the male (dihaploid inducer) parent, S. phureja. The importance of this for breeding programmes that use dihaploid potatoes is to some extent dependent upon whether the S. phureja DNA influences dihaploid morphology. In the present study, 21 characters were used to compare the morphology of six dihaploids with those of their parents: S. tuberosum (cvs `Pentland Dell' and `Pentland Crown') and S. phureja (IVP48). Characteristics of S. phureja were found in all of the dihaploids examined. In principal component analyses, dihaploids formed intermediate groupings positioned between those of the parents, although much closer to S. tuberosum. It is concluded there is evidence that DNA originating from the dihaploid inducer can affect the morphology of potato dihaploids. Implications of the findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050406
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  • 2
    Electronic Resource
    Electronic Resource
    Developing an appropriate strategy to assess genetic variability in plant germplasm collections (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1125-1131 
    Details
    ISSN: 1432-2242
    Keywords: Key words Lupin ; Lupinus albus ; Germplasm collection ; ISSR-PCR ; Microsatellite ; Genebank
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The value of molecular biology for monitoring the genetic status of germplasm collections is subject to practical limitations. The large number and variability of accessions held usually dictates the approach that can be employed. A quick, simple but reliable molecular protocol must be combined with an appropriate strategy for handling large sample sizes. In this study, ISSR-PCR was used to reveal genetic variability within and between accessions held in a collection of lupin germplasm. Pooling of DNA from individuals within accessions was found to be the most appropriate strategy for assessing large quantities of plant material. Band profiles generated from pools containing five individuals were fully representative of all constituent individuals used in the mix. Pools comprising 10 or 20 individuals, however, sometimes failed to contain minor bands that had been present only in the profile of one individual. Variation was observed between pools containing five different genotypes from the same accession. Routine large-scale screens are required to assess the genetic diversity and homogeneity of the lupin germplasm collection held in Reading. It is concluded that 2–3 pools of five genotypes may be sufficient to represent the genetic variability within and between accessions in the lupin and similar collections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051176
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    Paper (German National Licenses)
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